Blister artifact formation due to organic solvent effects on Spurr's epoxy resin

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dc.authorid0000-0002-4474-7371en_US
dc.contributor.authorKayton, Robert J.
dc.date.accessioned2024-07-12T21:02:08Z
dc.date.available2024-07-12T21:02:08Z
dc.date.issued1998en_US
dc.departmentFakülteler, Tıp Fakültesien_US
dc.description.abstractWrinkles and air bubble artifacts may occur when preparing slides of semithin sections (0.5 ?m) from blocks embedded in different resins. More than aesthetically annoying, wrinkles and air bubble artifacts may prohibit study of small structures. Present observations suggest that organic solvent based mounting media may interact with the resin of the section. This sometimes causes wrinkles and air bubble artifacts in the sections that degrade the quality of light microscope images. We compared the quality of semithin sections of several tissues in different resins using various types of mounting media. We observed that sections from Spurr's resin have many more artifacts. In particular, small, 2–10 ?m round or oblong blister-like artifacts often plague our Spurr's resin sections. We demonstrate that Spurr's resin sections react with toluene and xylene in organic solvent based mounting media forming blisters, while sections from Araldite and L. R. White do not. We suggest combinations of embedding and mounting media for successful preparation of semithin sections for light microscopy without wrinkles, blisters, or air bubble artifacts.en_US
dc.identifier.citationKayton, R.J. ve Aktas, R.G. (1998). Blister Artifact Formation Due to Organic Solvent Effects on Spurr’s Epoxy Resin Semi-thin Sections. Biotechnic&Histochemistry, Taylor and francis Online. 73(3), s. 157-163.en_US
dc.identifier.endpage163en_US
dc.identifier.issn1052-0295
dc.identifier.issn1473-7760
dc.identifier.issue3en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage157en_US
dc.identifier.urihttps://www.tandfonline.com/doi/abs/10.3109/10520299809140522
dc.identifier.urihttps://hdl.handle.net/20.500.12415/3585
dc.identifier.volume73en_US
dc.institutionauthorAktas, Ranan Gulhan
dc.language.isoenen_US
dc.publisherTaylor and Francis Onlineen_US
dc.relation.ispartofBiotechnic & Histochemistryen_US
dc.relation.isversionof10.3109/10520299809140522en_US
dc.relation.publicationcategoryUluslararası Hakemli Dergide Makale - Kurum Öğretim Elemanıen_US
dc.rightsCC0 1.0 Universal*
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/*
dc.snmzKY00429
dc.subjectartifactsen_US
dc.subjectepoxyen_US
dc.subjectlight microscopyen_US
dc.subjectmounting mediaen_US
dc.subjectplastic sectionsen_US
dc.subjectresinen_US
dc.subjectSpurr'sen_US
dc.subjecttolueneen_US
dc.subjectxyleneen_US
dc.titleBlister artifact formation due to organic solvent effects on Spurr's epoxy resinen_US
dc.typeArticle
dspace.entity.typePublication

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