Blister artifact formation due to organic solvent effects on Spurr's epoxy resin
Küçük Resim Yok
Tarih
1998
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor and Francis Online
Erişim Hakkı
CC0 1.0 Universal
info:eu-repo/semantics/openAccess
info:eu-repo/semantics/openAccess
Özet
Wrinkles and air bubble artifacts may occur when preparing slides of semithin sections (0.5 ?m) from blocks embedded in different resins. More than aesthetically annoying, wrinkles and air bubble artifacts may prohibit study of small structures. Present observations suggest that organic solvent based mounting media may interact with the resin of the section. This sometimes causes wrinkles and air bubble artifacts in the sections that degrade the quality of light microscope images. We compared the quality of semithin sections of several tissues in different resins using various types of mounting media. We observed that sections from Spurr's resin have many more artifacts. In particular, small, 2–10 ?m round or oblong blister-like artifacts often plague our Spurr's resin sections. We demonstrate that Spurr's resin sections react with toluene and xylene in organic solvent based mounting media forming blisters, while sections from Araldite and L. R. White do not. We suggest combinations of embedding and mounting media for successful preparation of semithin sections for light microscopy without wrinkles, blisters, or air bubble artifacts.
Açıklama
Anahtar Kelimeler
artifacts, epoxy, light microscopy, mounting media, plastic sections, resin, Spurr's, toluene, xylene
Kaynak
Biotechnic & Histochemistry
WoS Q Değeri
Scopus Q Değeri
Q2
Cilt
73
Sayı
3
Künye
Kayton, R.J. ve Aktas, R.G. (1998). Blister Artifact Formation Due to Organic Solvent Effects on Spurr’s Epoxy Resin Semi-thin Sections. Biotechnic&Histochemistry, Taylor and francis Online. 73(3), s. 157-163.
