Sperm selection and embryo development: a comparison of the density gradient centrifugation and microfluidic chip sperm preparation methods in patients with astheno-teratozoospermia

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dc.authorid0000-0003-2802-7793en_US
dc.authorid0000-0002-4605-1167en_US
dc.authorid0000-0002-0717-1756en_US
dc.contributor.authorSelam, Belgin
dc.contributor.authorÇağla, Güler
dc.contributor.authorMelil, Süreyya
dc.contributor.authorÖzekici, Ümit
dc.contributor.authorDönmez Çakıl, Yaprak
dc.contributor.authorCincik, Mehmet
dc.date.accessioned2024-07-12T21:05:21Z
dc.date.available2024-07-12T21:05:21Z
dc.date.issued2021en_US
dc.departmentFakülteler, Tıp Fakültesien_US
dc.description.abstractIn recent years, microfluidic chip-based sperm sorting has emerged as an alternative tool to centrifugation-based conventional techniques for in vitro fertilization. This prospective study aims to compare the effects of density gradient centrifugation and microfluidic chip sperm preparation methods on embryo development in patient populations with astheno-teratozoospermia. In the study, the semen samples of the patients were divided into two groups for preparation with either the microfluidic or density gradient methods. Selected spermatozoa were then used to fertilize mature sibling oocytes and the semen parameters and embryo development on days 3 and 5 were assessed. While the density gradient group was associated with a higher sperm concentration, motility (progressive and total) was significantly higher in the microfluidic chip group. No significant differences were observed in the fertilization rates or grade 1 (G1) and grade 2 (G2) proportions of the third-day embryos. Furthermore, while the proportions of the poor, fair and good blastocysts on day 5 did not differ significantly, excellent blastocysts (indicating high-quality embryos) were observed in a significantly higher proportion of the microfluidic chip group. When compared to the classical density gradient method, the microfluidic chip sperm preparation yielded sperm with higher motility and higher quality blastocysts at day 5; in patients with astheno-teratozoospermia.en_US
dc.identifier.citationGuler, C., Melil, S., Ozekici, U., Donmez Cakil, Y., Selam, B., ve Cincik, M. (2021).Sperm selection and embryo development: a comparison of the density gradient centrifugation and microfluidic chip sperm preparation methods in patients with astheno-teratozoospermia. Life, MDPI. 11(9).en_US
dc.identifier.doi10.3390/life11090933
dc.identifier.endpage9en_US
dc.identifier.issn2075-1729
dc.identifier.issue9en_US
dc.identifier.pmid34575082en_US
dc.identifier.scopus2-s2.0-85114841193en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://www.mdpi.com/2075-1729/11/9/933
dc.identifier.urihttps://doi.prg/10.3390/life11090933
dc.identifier.urihttps://hdl.handle.net/20.500.12415/3891
dc.identifier.volume11en_US
dc.identifier.wosWOS:000700237400001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorGüler, Çağla
dc.institutionauthorMelil, Süreyya
dc.institutionauthorÖzekici, Ümit
dc.institutionauthorDönmez Çakıl, Yaprak
dc.institutionauthorCincik, Mehmet
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.relation.ispartofLifeen_US
dc.relation.publicationcategoryUluslararası Hakemli Dergide Makale - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKY02193
dc.subjectmale infertilityen_US
dc.subjectastheno-teratozoospermiaen_US
dc.subjectmicrochipen_US
dc.subjectdensity gradient centrifugationen_US
dc.subjectembryoen_US
dc.subjectblastocysten_US
dc.titleSperm selection and embryo development: a comparison of the density gradient centrifugation and microfluidic chip sperm preparation methods in patients with astheno-teratozoospermiaen_US
dc.typeArticle
dspace.entity.typePublication

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