Different approaches for breast cancer: voltage gated potassium channels and micrornas

Basit Öğe Kaydı
dc.authorid0000-0003-3771-3277en_US
dc.contributor.authorÖner, Çağrı
dc.contributor.authorTurgut Coşan, Didem
dc.contributor.authorÇolak, Ertuğrul
dc.date.accessioned2024-07-12T21:03:04Z
dc.date.available2024-07-12T21:03:04Z
dc.date.issued2015en_US
dc.departmentFakülteler, Tıp Fakültesien_US
dc.description.abstractMicro RNAs and voltage-gated potassium channels (VGPCs) both play critical roles in the development of cancer. We aimed to reveal the diversity of miR-126/126*, which effects angiogenesis and vascular development through the inhibition of VGPCs. In this study, potassium channel inhibitors, including tetraethylammonium (5mM), 4-aminopyridine (5mM), margatoxin (1µM) and astemizole (2µM), were applied to MCF-7 and MDA-MB-231 breast cancer cell lines. After totally isolating RNA from the cells, Real-Time Polymerase Chain Reaction was used in order to identify gene expressions. OneWay ANOVA was used for variation analyses, while Tukey HSD and Tamhane were used to assess whether multiple comparisons were statistically significant (P < 0.001). Our results showed an increase in miR-126/126* expressions after the channel inhibition of MCF-7 and MDA-MB-231 cell lines (P < 0.001). miR-126/126* expressions were increased using TEA, 4-AP and astemizole in both cell lines. miR-126/126* expressions were only increased through the use of margatoxin in MCF-7. miR-126/126* may interact with voltage-gated potassium channels. In our study, the inhibition of K channels using K channel blockers resulted in an increase of miR-126/126* expression. Therefore, our data suggested that there could be another perspective between K channels and non-coding RNAs in the development of breast cancer.en_US
dc.identifier.citationÖner, Ç., Turgut Coşan, D. ve Çolak, E. (2015). Different approaches for breast cancer: voltage gated potassium channels and micrornas. Communications Faculty of Sciences University of Ankara Series C-Biology, Ankara University Faculty of Sciences. 24(1-2), s. 1-17.en_US
dc.identifier.endpage17en_US
dc.identifier.issn1303-6025
dc.identifier.issue1.Şuben_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://dergipark.org.tr/en/pub/communc/issue/60636/892863
dc.identifier.urihttps://hdl.handle.net/20.500.12415/3627
dc.identifier.volume24en_US
dc.institutionauthorÖner, Çağrı
dc.language.isoenen_US
dc.publisherAnkara University Faculty of Sciencesen_US
dc.relation.ispartofCommunications Faculty of Sciences University of Ankara Series C-Biologyen_US
dc.relation.isversionof10.1501/commuc_0000000181en_US
dc.relation.publicationcategoryUlusal Hakemli Dergide Makale - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKY00552
dc.subjectBreast Canceren_US
dc.subjectmiR-126/126*en_US
dc.subjectVoltage-Gated Potassium Channelsen_US
dc.titleDifferent approaches for breast cancer: voltage gated potassium channels and micrornasen_US
dc.typeArticle
dspace.entity.typePublication

Dosyalar

Koleksiyon

Tıp Fakültesi Koleksiyonu