Different approaches for breast cancer: voltage gated potassium channels and micrornas
Küçük Resim Yok
Tarih
2015
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Ankara University Faculty of Sciences
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Micro RNAs and voltage-gated potassium channels (VGPCs) both play critical roles in the development of cancer. We aimed to reveal the diversity of miR-126/126*, which effects angiogenesis and vascular development through the inhibition of VGPCs. In this study, potassium channel inhibitors, including tetraethylammonium (5mM), 4-aminopyridine (5mM), margatoxin (1µM) and astemizole (2µM), were applied to MCF-7 and MDA-MB-231 breast cancer cell lines. After totally isolating RNA from the cells, Real-Time Polymerase Chain Reaction was used in order to identify gene expressions. OneWay ANOVA was used for variation analyses, while Tukey HSD and Tamhane were used to assess whether multiple comparisons were statistically significant (P < 0.001). Our results showed an increase in miR-126/126* expressions after the channel inhibition of MCF-7 and MDA-MB-231 cell lines (P < 0.001). miR-126/126* expressions were increased using TEA, 4-AP and astemizole in both cell lines. miR-126/126* expressions were only increased through the use of margatoxin in MCF-7. miR-126/126* may interact with voltage-gated potassium channels. In our study, the inhibition of K channels using K channel blockers resulted in an increase of miR-126/126* expression. Therefore, our data suggested that there could be another perspective between K channels and non-coding RNAs in the development of breast cancer.
Açıklama
Anahtar Kelimeler
Breast Cancer, miR-126/126*, Voltage-Gated Potassium Channels
Kaynak
Communications Faculty of Sciences University of Ankara Series C-Biology
WoS Q Değeri
Scopus Q Değeri
Cilt
24
Sayı
1.Şub
Künye
Öner, Ç., Turgut Coşan, D. ve Çolak, E. (2015). Different approaches for breast cancer: voltage gated potassium channels and micrornas. Communications Faculty of Sciences University of Ankara Series C-Biology, Ankara University Faculty of Sciences. 24(1-2), s. 1-17.
