Long-term characteristics of human-derived biliary organoids under a single continuous culture condition

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dc.authorid0000-0002-4474-7371en_US
dc.contributor.authorKarski, Michael
dc.contributor.authorIssac, Biju
dc.contributor.authorSun, Liang
dc.contributor.authorRockowitz, Shira
dc.contributor.authorSliz, Piotr
dc.contributor.authorVakili, Khashayar
dc.contributor.authorAktaş, Ranan G.
dc.date.accessioned2024-07-12T21:02:59Z
dc.date.available2024-07-12T21:02:59Z
dc.date.issued2022en_US
dc.departmentFakülteler, Tıp Fakültesien_US
dc.description.abstractOrganoids have been used to investigate the three-dimensional (3D) organization and function of their respective organs. These self-organizing 3D structures offer a distinct advantage over traditional two-dimensional (2D) culture techniques by creating a more physiologically relevant milieu to study complex biological systems. The goal of this study was to determine the feasibility of establishing organoids from various pediatric liver diseases and characterize the long-term evolution of cholangiocyte organoids (chol-orgs) under a single continuous culture condition. We established chol-orgs from 10 different liver conditions and characterized their multicellular organization into complex epithelial structures through budding, merging, and lumen formation. Immunofluorescent staining, electron microscopy, and single-nucleus RNA (snRNA-seq) sequencing confirmed the cholangiocytic nature of the chol-orgs. There were significant cell population differences in the transcript profiles of two-dimensional and organoid cultures based on snRNA-seq. Our study provides an approach for the generation and long-term maintenance of chol-orgs from various pediatric liver diseases under a single continuous culture condition.en_US
dc.identifier.citationAktas, R.G., Karski, M., Issac, B. and at all. (2022). Long-term characteristics of human-derived biliary organoids under a single continuous culture condition. Cells, 11, p.2073-4409.en_US
dc.identifier.doi10.3390/cells11233797
dc.identifier.endpage4409en_US
dc.identifier.pmid36497057en_US
dc.identifier.scopus2-s2.0-85143620447en_US
dc.identifier.startpage2073en_US
dc.identifier.urihttps://doi.prg/10.3390/cells11233797
dc.identifier.urihttps://hdl.handle.net/20.500.12415/3606
dc.identifier.volume11en_US
dc.identifier.wosWOS:000897408300001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAktaş, Ranan G.
dc.language.isoenen_US
dc.publisherCellsen_US
dc.relation.ispartofCellsen_US
dc.relation.publicationcategoryUluslararası Hakemli Dergide Makale - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKY00488
dc.subject3Den_US
dc.subjectBiliary systemen_US
dc.subjectExtracellular matrixen_US
dc.subjectMicroenvironmenten_US
dc.subjectOrganoiden_US
dc.subjectPrimary cellsen_US
dc.subjectThree dimensionalen_US
dc.titleLong-term characteristics of human-derived biliary organoids under a single continuous culture conditionen_US
dc.typeArticle
dspace.entity.typePublication

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