Ultrastructural immunolocalization of basic fibroblast growth factor in fibroblasts and extracellular matrix

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dc.authorid0000-0002-4474-7371en_US
dc.contributor.authorKayton, Robert J.
dc.date.accessioned2024-07-12T21:04:03Z
dc.date.available2024-07-12T21:04:03Z
dc.date.issued2000en_US
dc.departmentFakülteler, Tıp Fakültesien_US
dc.description.abstractBasic fibroblast growth factor (bFGF) is thought to play an important role in normal tissue repair and wound healing. It is a potent mitogenic and chemotactic factor for fibroblasts, regulating proliferation and extracellular matrix (ECM) production by these cells. In this study, we present morphologic evidence of the ultrastructural location of bFGF in fibroblasts and ECM using several antibodies, tissues, and species. Distinct labeling is seen in the nuclei of fibroblasts and some labeling in the cytosol. Immunolabeling of the cytosol excludes organelles involved in the usual secretory pathway, such as rough endoplasmic reticulum, Golgi apparatus, and secretory vacuoles. The same labeling is observed with either polyclonal or monoclonal antibodies. We suggest that bFGF functions as a nuclear protein in fibroblasts and is not secreted by a normal secretory pathway. Fibroblasts may export bFGF via unique cellular pathways that are clearly distinct from classic signal peptide mediated secretion. This may provide a source for ECM-resident bFGF. The same antibodies show different labeling intensity in the ECM. This protein, through integration into the ECM, may act as a local regulator and promote regeneration of these tissues after wounding. Direct evidence is the dramatic reduction of bFGF labeling in axotomized rat ECM collagen fibers versus control animals.en_US
dc.identifier.citationAktas, R. ve Kayton, R. (2000). Ultrastructural immunolocalization of basic fibroblast growth factor in fibroblasts and extracellular matrix. Histochemistry and Cell Biology, Elsevier. 113, s. 227–233.en_US
dc.identifier.endpage233en_US
dc.identifier.issn0948-6143
dc.identifier.issn1432-119X
dc.identifier.issue113en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage227en_US
dc.identifier.urihttps://link.springer.com/article/10.1007/s004180050442#citeas
dc.identifier.urihttps://hdl.handle.net/20.500.12415/3748
dc.institutionauthorAktas, Ranan Gulhan
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofHistochemistry and Cell Biologyen_US
dc.relation.isversionof10.1007/s004180050442en_US
dc.relation.publicationcategoryUluslararası Hakemli Dergide Makale - Kurum Öğretim Elemanıen_US
dc.rightsCC0 1.0 Universal*
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/*
dc.snmzKY01189
dc.subjectCytosolen_US
dc.subjectCollagen Fiberen_US
dc.subjectGolgi Apparatusen_US
dc.subjectSecretory Pathwayen_US
dc.subjectBasic Fibroblast Growth Factoren_US
dc.titleUltrastructural immunolocalization of basic fibroblast growth factor in fibroblasts and extracellular matrixen_US
dc.typeArticle
dspace.entity.typePublication

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