Ultrastructural immunolocalization of basic fibroblast growth factor in fibroblasts and extracellular matrix
Küçük Resim Yok
Tarih
2000
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier
Erişim Hakkı
CC0 1.0 Universal
info:eu-repo/semantics/openAccess
info:eu-repo/semantics/openAccess
Özet
Basic fibroblast growth factor (bFGF) is thought to play an important role in normal tissue repair and wound healing. It is a potent mitogenic and chemotactic factor for fibroblasts, regulating proliferation and extracellular matrix (ECM) production by these cells. In this study, we present morphologic evidence of the ultrastructural location of bFGF in fibroblasts and ECM using several antibodies, tissues, and species. Distinct labeling is seen in the nuclei of fibroblasts and some labeling in the cytosol. Immunolabeling of the cytosol excludes organelles involved in the usual secretory pathway, such as rough endoplasmic reticulum, Golgi apparatus, and secretory vacuoles. The same labeling is observed with either polyclonal or monoclonal antibodies. We suggest that bFGF functions as a nuclear protein in fibroblasts and is not secreted by a normal secretory pathway. Fibroblasts may export bFGF via unique cellular pathways that are clearly distinct from classic signal peptide mediated secretion. This may provide a source for ECM-resident bFGF. The same antibodies show different labeling intensity in the ECM. This protein, through integration into the ECM, may act as a local regulator and promote regeneration of these tissues after wounding. Direct evidence is the dramatic reduction of bFGF labeling in axotomized rat ECM collagen fibers versus control animals.
Açıklama
Anahtar Kelimeler
Cytosol, Collagen Fiber, Golgi Apparatus, Secretory Pathway, Basic Fibroblast Growth Factor
Kaynak
Histochemistry and Cell Biology
WoS Q Değeri
Scopus Q Değeri
Q1
Cilt
Sayı
113
Künye
Aktas, R. ve Kayton, R. (2000). Ultrastructural immunolocalization of basic fibroblast growth factor in fibroblasts and extracellular matrix. Histochemistry and Cell Biology, Elsevier. 113, s. 227–233.
