Rescue IVM of denuded GV- and MI-stage oocytes of premenopausal rats with oncostatin M, insulin-like growth factor I, and growth hormone

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dc.authorid0000-0002-4605-1167en_US
dc.authorid0000-0001-5114-8660en_US
dc.authorid0000-0002-0717-1756en_US
dc.contributor.authorAkdemir, Yeşim
dc.contributor.authorSelam, Belgin
dc.contributor.authorDönmez Çakıl, Yaprak
dc.contributor.authorSitar, Mustafa Erinç
dc.contributor.authorCincik, Mehmet
dc.date.accessioned2024-07-12T21:05:22Z
dc.date.available2024-07-12T21:05:22Z
dc.date.issued2022en_US
dc.departmentFakülteler, Tıp Fakültesien_US
dc.description.abstractImmature oocytes are retrieved and matured through in vitro maturation (IVM). Maturation, fertilization rates, and embryo development via IVM are all lower than those found in vitro fertilization (IVF) cycles. We investigated the effects of oncostatin M (OSM), insulin-like growth factor-1 (IGF-I), and growth hormone (GH) in rescue IVM. A total of 111 germinal vesicle (GV) and 17 metaphase I (MI) oocytes were obtained after conventional IVF from 28 female Wistar albino rats. Denuded immature oocytes were cultured in maturation medium supplemented with OSM, IGF-1, or GH. The quantities of metaphase II (MII) oocytes matured from the GV stage were 17 of 30 (56.6%), 15 of 28 (53.5%), 10 of 30 (33.3%), and 7 of 23 (30.3%), in control, OSM, IGF-I, and GH groups, respectively. Maturation rates in control and OSM groups were higher than those in IGF-I and GH groups (p = 0.001). The quantities of MII oocytes matured from MI stage were 7 of 7 (100%), 4 of 4 (100%), 1 of 1 (100%), and 1 of 5 (20%) in control, OSM, IGF-I, and GH groups, respectively. Maturation rates from MI to MII stages in control, OSM, and IGF-I groups were higher than those in the GH group (p = 0.004). Acceptable maturation rates are observed with OSM in rat oocytes in rescue IVM.en_US
dc.identifier.citationAkdemir, Y., Donmez Cakil, Y., Selam, B., Sitar, M. E. ve Cincik, M. (2022). Rescue IVM of denuded GV- and MI-stage oocytes of premenopausal rats with oncostatin M, insulin-like growth factor I, and growth hormone. Life, MDPI. 12(8).en_US
dc.identifier.doi10.3390/life12081247
dc.identifier.endpage10en_US
dc.identifier.issn2075-1729
dc.identifier.issue8en_US
dc.identifier.pmid36013426en_US
dc.identifier.scopus2-s2.0-85137333314en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://www.mdpi.com/2075-1729/12/8/1247
dc.identifier.urihttps://doi.prg/10.3390/life12081247
dc.identifier.urihttps://hdl.handle.net/20.500.12415/3892
dc.identifier.volume12en_US
dc.identifier.wosWOS:000845497200001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorDönmez Çakıl, Yaprak
dc.institutionauthorSitar, Mustafa Erinç
dc.institutionauthorCincik, Mehmet
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.relation.ispartofLifeen_US
dc.relation.publicationcategoryUluslararası Hakemli Dergide Makale - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKY02194
dc.subjectin-vitro maturationen_US
dc.subjectgerminal vesicleen_US
dc.subjectoncostatin Men_US
dc.subjectinsulin-like growth factor-1en_US
dc.subjectgrowth hormoneen_US
dc.subjectMII oocyteen_US
dc.subjectrescue in vitro maturationen_US
dc.titleRescue IVM of denuded GV- and MI-stage oocytes of premenopausal rats with oncostatin M, insulin-like growth factor I, and growth hormoneen_US
dc.typeArticle
dspace.entity.typePublication

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