Evaluation of the effect of diclofenac sodium and 5-fluourasil in a 3d cholesteatoma cell culture model

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dc.authorid0000-0001-7914-2201en_US
dc.contributor.authorKara, Ahmet
dc.contributor.authorDuman, Busra Oncel
dc.contributor.authorYazır, Yusufhan
dc.contributor.authorYılmaz, Mahmut Sinan
dc.contributor.authorUnal Halbutogulları, Zehra Seda
dc.contributor.authorDemir, Deniz
dc.contributor.authorÖztaş Kara, Rabia
dc.contributor.authorBayraktar, Hakan
dc.contributor.authorGuven, Mehmet
dc.contributor.authorDemir, Deniz
dc.date.accessioned2024-07-12T21:01:01Z
dc.date.available2024-07-12T21:01:01Z
dc.date.issued2019en_US
dc.departmentFakülteler, Tıp Fakültesien_US
dc.description.abstractIntroduction: Middle ear cholesteatoma is a benign disease with invasive and destructive clinical behaviors. It increases the rate of both chronic otitis media complications and revision surgeries. The most effective treatment of middle ear cholesteatoma is surgical excision, and there is no medical treatment for this disease. Exploring new medical treatment options may help to create treatment alternatives instead of surgery. Materials and Methods: Required cholesteatoma tissues for cell culture were excised from 4 different participants who underwent surgery in our clinic and agreed to give tissue for the study. Cholesteatoma-derived keratinocytes and fibroblasts were cocultured in temperature-sensitive culture dishes to make a three-dimensional (3D) cholesteatoma model. Then, the effects of 1% and 2% diclofenac sodium on viability and cell proliferation rates were examined using WST-1 and annexin-V tests. Results: Cell viability and proliferation rates were found to be lower and apoptosis rates were higher in the diclofenac sodium group versus the negative and positive control groups. Conclusion: In this present study, we described a new 3D cholesteatoma cell culture model developed using cell sheet technology and demonstrated the efficacy of diclofenac sodium on cholesteatoma for the first time in the literature. It may be used in patients with chronic otitis media with cholesteatoma, but further studies investigating ototoxic and neurotoxic effects of this molecule are needed.en_US
dc.identifier.citationKara, A., Duman, B.Ö., Yazır, Y., Yılmaz, M.S., Unal Halbutogulları, Z.S., Demir, D. vd. (2019). Evaluation of the effect of diclofenac sodium and 5-fluourasil in a 3d cholesteatoma cell culture model. Otology & Neurotology, Wolters Kluwer. 40(8), s. 1018-1025.en_US
dc.identifier.endpage1025en_US
dc.identifier.issn1537-4505
dc.identifier.issue8en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1018en_US
dc.identifier.urihttps://journals.lww.com/otology-neurotology/Fulltext/2019/09000/Evaluation_of_the_Effect_of_Diclofenac_Sodium_and.11.aspx
dc.identifier.urihttps://hdl.handle.net/20.500.12415/3439
dc.identifier.volume40en_US
dc.institutionauthorDemir, Deniz
dc.language.isoenen_US
dc.publisherWolters Kluweren_US
dc.relation.ispartofOtology & Neurotologyen_US
dc.relation.isversionof10.1097/MAO.0000000000002297en_US
dc.relation.publicationcategoryUluslararası Hakemli Dergide Makale - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKY03187
dc.subjectCell culture techniquesen_US
dc.subjectCholesteatomaen_US
dc.subjectDiclofenacen_US
dc.subjectFluorouracilen_US
dc.subjectTherapyen_US
dc.titleEvaluation of the effect of diclofenac sodium and 5-fluourasil in a 3d cholesteatoma cell culture modelen_US
dc.typeArticle
dspace.entity.typePublication

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