Evaluation of the effect of diclofenac sodium and 5-fluourasil in a 3d cholesteatoma cell culture model
Küçük Resim Yok
Tarih
2019
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wolters Kluwer
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Introduction: Middle ear cholesteatoma is a benign disease with invasive and destructive clinical behaviors. It increases the rate of both chronic otitis media complications and revision surgeries. The most effective treatment of middle ear cholesteatoma is surgical excision, and there is no medical treatment for this disease. Exploring new medical treatment options may help to create treatment alternatives instead of surgery. Materials and Methods: Required cholesteatoma tissues for cell culture were excised from 4 different participants who underwent surgery in our clinic and agreed to give tissue for the study. Cholesteatoma-derived keratinocytes and fibroblasts were cocultured in temperature-sensitive culture dishes to make a three-dimensional (3D) cholesteatoma model. Then, the effects of 1% and 2% diclofenac sodium on viability and cell proliferation rates were examined using WST-1 and annexin-V tests. Results: Cell viability and proliferation rates were found to be lower and apoptosis rates were higher in the diclofenac sodium group versus the negative and positive control groups. Conclusion: In this present study, we described a new 3D cholesteatoma cell culture model developed using cell sheet technology and demonstrated the efficacy of diclofenac sodium on cholesteatoma for the first time in the literature. It may be used in patients with chronic otitis media with cholesteatoma, but further studies investigating ototoxic and neurotoxic effects of this molecule are needed.
Açıklama
Anahtar Kelimeler
Cell culture techniques, Cholesteatoma, Diclofenac, Fluorouracil, Therapy
Kaynak
Otology & Neurotology
WoS Q Değeri
Scopus Q Değeri
Q1
Cilt
40
Sayı
8
Künye
Kara, A., Duman, B.Ö., Yazır, Y., Yılmaz, M.S., Unal Halbutogulları, Z.S., Demir, D. vd. (2019). Evaluation of the effect of diclofenac sodium and 5-fluourasil in a 3d cholesteatoma cell culture model. Otology & Neurotology, Wolters Kluwer. 40(8), s. 1018-1025.
