Evaluation of MALDI-TOF MS for identification of nontuberculous mycobacteria isolated from clinical specimens in mycobacteria growth indicator tube medium

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dc.contributor.authorGenc, Gonca Erkose
dc.contributor.authorDemir, Melda
dc.contributor.authorYaman, Gorkem
dc.contributor.authorKayar, Begum
dc.contributor.authorKoksal, Fatih
dc.contributor.authorSatana, Dilek
dc.date.accessioned2024-07-12T21:52:28Z
dc.date.available2024-07-12T21:52:28Z
dc.date.issued2018en_US
dc.departmentMaltepe Üniversitesien_US
dc.description.abstractNowadays, there is a rising worldwide incidence of diseases caused by nontuberculous mycobacteria (NTM) species, especially in immunocompromised patients and those with underlying chronic pulmonary diseases. Recently, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) became a method of choice for the identification of NTM species. The aim of this study was to evaluate MALDI-TOF MS for the identification of NTM isolates compared to the PCR-restriction enzyme analysis (PRA)-hsp65 method. In this study; a total of 152 NTM strains isolated from various clinical specimens were retrospectively analysed. MALDI-TOF MS successfully identified 148 (97.4%) of the 152 NTM isolates but failed to identify four (2.6%) of them. Bruker mycobacteria library gave spectral scores higher than 2.0 for 45 (29.6%) of NTM isolates, between 1.6 and 2.0 for 98 (64.5%) of NTM isolates, and lower than 1.6 for nine (5.9%) NTM isolates. The discordant results between MALDI-TOF MS and PRA-hsp65 analysis were confirmed by sequence analysis. In conclusion, MALDI-TOF MS is a technique capable of performing accurate, rapid, cost-effective, and easy identification of NTM isolates.en_US
dc.description.sponsorshipScientific Research Project Coordination Unit of Istanbul University [BEK-2017-25721, TYL-2016-20561]en_US
dc.description.sponsorshipThis work was supported by Scientific Research Project Coordination Unit of Istanbul University. Project number BEK-2017-25721, and TYL-2016-20561.en_US
dc.identifier.endpage219en_US
dc.identifier.issn1121-7138
dc.identifier.issue3en_US
dc.identifier.pmid29874386en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage214en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12415/8379
dc.identifier.volume41en_US
dc.identifier.wosWOS:000443556900006en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherEDIZIONI INT SRLen_US
dc.relation.ispartofNEW MICROBIOLOGICAen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzKY03106
dc.subjectNontuberculous mycobacteriaen_US
dc.subjectMALDI-TOF MSen_US
dc.subjectIdentificationen_US
dc.subjectPRA-hsp65en_US
dc.titleEvaluation of MALDI-TOF MS for identification of nontuberculous mycobacteria isolated from clinical specimens in mycobacteria growth indicator tube mediumen_US
dc.typeArticle
dspace.entity.typePublication

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