Subcellular distribution of basic fibroblast growth factor-like molecules ın fibroblasts and extracellular matrix
Küçük Resim Yok
Tarih
1999
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Cambridge University Press
Erişim Hakkı
CC0 1.0 Universal
info:eu-repo/semantics/openAccess
info:eu-repo/semantics/openAccess
Özet
Basic fibroblast growth factor (bFGF) is thought to play an important role in normal tissue repair and wound healing. It is a potent mitogenic and chemotactic factor for fibroblasts. To some degree bFGF regulates proliferation and extracellular matrix (ECM) production by these cells. In this study we investigate (i) the distribution of bFGF in fibroblasts and ECM of supporting tissues (ii) the subcellular location of this protein in vivo and, (iii) mechanisms for release of this growth factor from fibroblasts. Spurr's resin embedded ultrathin sections from adult rat sciatic nerve, adrenal gland, kidney; and from normal human lung and skin were labeled with either polyclonal (F3393-Sigma) or monoclonal antibodies (F6162-Sigma, C3316-ZymoGenetics Inc.) specific for bFGF using a two-step immunogold labeling method. Controls were incubated with either an equivalent volume of blocking solution (omitting the primary antibody) or an irrelevant antibody (Factor VIII, anti-Histamine)(Fig. 4).
Açıklama
Anahtar Kelimeler
Kaynak
Microscopy and Microanalysis
WoS Q Değeri
Scopus Q Değeri
Q3
Cilt
5
Sayı
2
Künye
Aktas, R. G. and Kayton, R. J. (1999). Subcellular Distribution Of Basic Fibroblast Growth Factor-Like Molecules In Fibroblasts And Extracellular Matrix. Microscopy and Microanalysis, Cambridge University Press. 5(S2), s. 1332–1333.
