Oxidative DNA base damage and base excision repair abnormalities in siblings of individuals with bipolar disorder

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dc.authorid0000-0002-0880-8974en_US
dc.authorid0000-0003-0632-1617en_US
dc.authorid0000-0002-7376-9545en_US
dc.contributor.authorÇelik, Hidayet Ece
dc.contributor.authorYılmaz, Seda
dc.contributor.authorKendirlioğlu, Burcu Kök
dc.contributor.authorÇörekli, Esma
dc.contributor.authorBekar, Nazlı Dal
dc.contributor.authorÇelik, Ömer
dc.contributor.authorYorguner, Neşe
dc.contributor.authorÖztürk, Bilge Targıtay
dc.contributor.authorİşlekel, Hüray
dc.contributor.authorÖzerdem, Ayşegül
dc.contributor.authorAkan, Pınar
dc.contributor.authorCeylan, Deniz
dc.contributor.authorTuna, Gamze
dc.date.accessioned2024-07-12T21:11:02Z
dc.date.available2024-07-12T21:11:02Z
dc.date.issued2023en_US
dc.departmentFakülteler, Tıp Fakültesien_US
dc.description.abstractPrevious evidence suggests elevated levels of oxidative DNA damage, particularly 8-hydroxy-2'- deoxyguanosine (8-OH-dG), and abnormalities in the repair of 8-OH-dG by the base excision repair (BER) in BD. However, the genetic disposition of these abnormalities remains unknown. In this study, we aimed to investigate the levels of oxidative DNA damage and BER mechanisms in individuals with BD and their siblings, as compared to healthy controls (HCs). 46 individuals with BD, 41 siblings of individuals with BD, and 51 HCs were included in the study. Liquid chromatography-tandem mass spectrometry was employed to evaluate the levels of 8-OH-dG in urine, which were then normalized based on urine creatinine levels. The real-time-polymerase chain reaction was used to measure the expression levels of 8-oxoguanine DNA glycosylase 1 (OGG1), apurinic/apyrimidinic endonuclease 1 (APE1), poly ADP-ribose polymerase 1 (PARP1), and DNA polymerase beta (POL?). The levels of 8-OH-dG were found to be elevated in both individuals with BD and their siblings when compared to the HCs. The OGG1 and APE1 expressions were downregulated, while POL? expressions were upregulated in both the patient and sibling groups compared to the HCs. Age, smoking status, and the number of depressive episodes had an impact on APE1 expression levels in the patient group while body mass index, smoking status, and past psychiatric history had an impact on 8-OH-dG levels in siblings. Both individuals with BD and unaffected siblings presented similar abnormalities regarding oxidative DNA damage and BER, suggesting a link between abnormalities in DNA damage / BER mechanisms and familial susceptibility to BD. Our findings suggest that targeting the oxidative DNA damage and BER pathway could offer promising therapeutic strategies for reducing the risk of age-related diseases and comorbidities in individuals with a genetic predisposition to BD.en_US
dc.identifier.citationÇelik, H.E., Yılmaz, S., Akşahin, İ. and at all. (2023). Oxidative DNA base damage and base excision repair abnormalities in siblings of individuals with bipolar disorder. Research Square, p.1-22.en_US
dc.identifier.endpage22en_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12415/4319
dc.institutionauthorÇelik, Hidayet Ece
dc.institutionauthorKendirlioğlu, Burcu Kök
dc.institutionauthorÇörekli, Esma
dc.language.isoenen_US
dc.publisherResearch Squareen_US
dc.relation.ispartofResearch Squareen_US
dc.relation.isversionof10.21203/rs.3.rs-3273378/v1en_US
dc.relation.publicationcategoryUluslararası Hakemli Dergide Makale - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKY03344
dc.subjectBipolar disorderen_US
dc.subjectSiblingen_US
dc.subjectHigh risken_US
dc.subjectOxidative DNA damageen_US
dc.subjectDNA repairen_US
dc.titleOxidative DNA base damage and base excision repair abnormalities in siblings of individuals with bipolar disorderen_US
dc.typeArticle
dspace.entity.typePublication

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