Investigation of the effect of dipyrone on cells isolated from intervertebral disc tissue

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dc.authoridAkyuva, Yener/0000-0001-8171-5929en_US
dc.authoridYILMAZ, Ibrahim/0000-0003-2003-6337en_US
dc.authoridÖzbek, hanefi/0000-0002-8084-7855en_US
dc.contributor.authorAkgün, Feride Sinem
dc.contributor.authorSirin, Duygu Yasar
dc.contributor.authorYılmaz, Ibrahim
dc.contributor.authorKaraarslan, Numan
dc.contributor.authorÖzbek, Hanefi
dc.contributor.authorSimsek, Abdullah Talha
dc.contributor.authorKaya, Yasin Emre
dc.date.accessioned2024-07-12T21:37:17Z
dc.date.available2024-07-12T21:37:17Z
dc.date.issued2019en_US
dc.department[Belirlenecek]en_US
dc.description.abstractThe present study aimed to evaluate the effects of dipyrone, an indispensable analgesic, anti-pyretic and anti-spasmodic used in emergency departments, on nucleus pulposus and annulus fibrosus cells in vitro. After surgical biopsy, primary cell cultures were prepared from intact intervertebral disc tissues. Dipyrone was administered to the cultures in the experimental groups except for the control group. The data obtained were statistically evaluated. The proliferation was identified to be suppressed via MTT analysis. The gene expression profile of the intervertebral disc cells in the dipyrone-treated groups was significantly changed. The expression of chondroadherin, cartilage oligo matrix protein, interleukin-1 beta and metalloproteinase (MMP)-19 genes were decreased, but MMP-13 and MMP-7 genes expressions were increased, as determined via reverse transcription-quantitative PCR. AO/PI staining revealed that no apoptotic or other type of cell death was detectable after administration of dipyrone does not mean that the drug is innocuous. The occurrence of cellular senescence and/or the halt of cell proliferation may also be important mechanisms underlying the adverse inhibitory effects of dipyrone. Therefore, prior to administering dipyrone in clinical practice, all possible adverse effects of this drug should be considered.en_US
dc.identifier.doi10.3892/etm.2019.7576
dc.identifier.endpage224en_US
dc.identifier.issn1792-0981
dc.identifier.issn1792-1015
dc.identifier.issue1en_US
dc.identifier.pmid31258656en_US
dc.identifier.startpage216en_US
dc.identifier.urihttps://doi.org/10.3892/etm.2019.7576
dc.identifier.urihttps://hdl.handle.net/20.500.12415/6732
dc.identifier.volume18en_US
dc.identifier.wosWOS:000476606000026en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherSpandidos Publ Ltden_US
dc.relation.ispartofExperimental And Therapeutic Medicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKY04074
dc.subjectAnnulus Fibrosusen_US
dc.subjectDipyroneen_US
dc.subjectIntervertebral Disc Tissueen_US
dc.subjectMetamizoleen_US
dc.subjectNucleus Pulposusen_US
dc.subjectPrimary Cell Cultureen_US
dc.titleInvestigation of the effect of dipyrone on cells isolated from intervertebral disc tissueen_US
dc.typeArticle
dspace.entity.typePublication

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