4-hydroxycoumarin effects on both cellular and genetic characteristics of hepatocellular carcinoma cells

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dc.authorid0000-0003-3771-3277en_US
dc.contributor.authorÖner, Çağrı
dc.contributor.authorSoyergin, Dilara
dc.contributor.authorÖzyurt, Ahmet
dc.contributor.authorÇolak, Ertuğrul
dc.date.accessioned2024-07-12T21:03:10Z
dc.date.available2024-07-12T21:03:10Z
dc.date.issued2022en_US
dc.departmentFakülteler, Tıp Fakültesien_US
dc.description.abstract4-Hydroxycoumarin is an aromatic substance which is metabolized in liver and used as a therapeutic agent for various diseases. We aimed to determine the impact of 4-Hydroxycoumarin on HepG2 cells according to their viability, proliferation, adhesion and gene expression of cellular behavior parameters. Inhibitory concentration 50 (IC50) of 4-Hydroxycoumarin was detected on HepG2 cells. After determining the optimal time and concentration, the effect of 4-Hydroxycoumarin on viability, proliferation and adhesion of HepG2 cells were observed. Gene expressions of Ki-67, MMP-2, MMP-9 and piR-823 expression were determined by using Real Time-Polymerase Chain Reaction. IC50 value of 4-Hydroxycoumarin on HepG2 cells was 5 ?M at the 48 h (p < 0.001). 5 ?M at the 48 h of 4-Hydroxycoumarin caused to decrease of proliferation (p < 0.001) and viability of HepG2 cells (p < 0.001). Viability rate were supported by hematoxylin-eosin staining. Adhesion of cells increased on 4-Hydroxycoumarin treated cells compared to control (p < 0.001). While Ki-67 gene expression of 4-Hydroxycoumarin treated group decreased (p < 0.001); upregulation of MMP-2, MMP-9 and piR-823 expressions were determined in 4-Hydroxycoumarin treated group (p < 0.001). According to the cellular and genetic perspective, 4-Hydroxycoumarin might be useful to treat hepatocellular carcinoma. High adhesion and proliferation are the main characteristics of HepG2 cells, 4-Hydroxycoumarin treatment caused to lose these functions. The genetic markers of these characteristics also supported the same result. These are first findings about the effect of 4-Hydroxycoumarin on piR-823 and genes which are key features of cellular survival mechanisms.en_US
dc.identifier.citationÖner, Ç., Soyergin, D., Özyurt, A. ve Çolak, E. (2022). 4-hydroxycoumarin effects on both cellular and genetic characteristics of hepatocellular carcinoma cells. Cytology and Genetics, Springerlink. 56, s. 292-300.en_US
dc.identifier.doi10.3103/S0095452722030094
dc.identifier.endpage300en_US
dc.identifier.issn1934-9440
dc.identifier.scopus2-s2.0-85132278087en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage292en_US
dc.identifier.urihttps://link.springer.com/article/10.3103/S0095452722030094
dc.identifier.urihttps://doi.prg/10.3103/S0095452722030094
dc.identifier.urihttps://hdl.handle.net/20.500.12415/3641
dc.identifier.volume56en_US
dc.identifier.wosWOS:000812257100011en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorÖner, Çağrı
dc.institutionauthorSoyergin, Dilara
dc.institutionauthorÖzyurt, Ahmet
dc.language.isoenen_US
dc.publisherSpringerlinken_US
dc.relation.ispartofCytology and Geneticsen_US
dc.relation.publicationcategoryUlusal Hakemli Dergide Makale - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKY00601
dc.subject4-Hydroxycoumarinen_US
dc.subjectmatrix metallo proteinaseen_US
dc.subjectpiRNAen_US
dc.subjectpiR-823en_US
dc.subjecthepatocellular carcinomaen_US
dc.title4-hydroxycoumarin effects on both cellular and genetic characteristics of hepatocellular carcinoma cellsen_US
dc.typeArticle
dspace.entity.typePublication

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