Are Intervertebral Disc Tissue Cells Damaged When Attempting to Prevent Thrombus Formation Using Dabigatran, A New Oral Anticoagulant?

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dc.authorid0000-0001-6537-866Xen_US
dc.contributor.authorKaplan, Necati
dc.contributor.authorKaraarslan, Numan
dc.contributor.authorYilmaz, Ibrahim
dc.contributor.authorYasar Sirin, Duygu
dc.contributor.authorAkgun, Feride Sinem
dc.contributor.authorCaliskan, Tezcan
dc.contributor.authorSimsek, Abdullah Talha
dc.contributor.authorOzbek, Hanefi
dc.date.accessioned2024-07-12T21:56:41Z
dc.date.available2024-07-12T21:56:41Z
dc.date.issued2019en_US
dc.departmentMaltepe Üniversitesien_US
dc.description.abstractAIM: To investigate the effect of dabigatran, a new oral anticoagulant, on human primary cell cultures isolated from intact intervertebral disc tissue. MATERIAL and METHODS: Cell cultures were prepared from tissues obtained from six cases who had undergone surgery due to spinal trauma. Dabigatran, an active pharmacological agent, was applied to intact annulus fibrosus (AF)/nucleus pulposus (NP) primary cell cultures from the study group. After performing cell viability, toxicity, and proliferation tests on all cultures in the control and study groups, the surface morphologies of the samples were evaluated. Subsequently, chondroadherin (CHAD), cartilage oligomeric matrix protein (COMP), and matrix metalloproteinase (MMP)-13 and -19 expressions were measured via a real-time polymerase chain reaction (RT-PCR). Data were analyzed statistically. RESULTS: In the proliferation assays performed on the 20th day of the study, cells in the dabigatran-supplemented group were reported to have lost 46.37% more viability than those in the control group. Expressions of all genes examined except MMP-13 were evaluated in the control group by time, but in contrast to the control group results, COMP and MMP-19 gene expressions decreased in the dabigatran-treated group. No CHAD or MMP-13 expression was noted in these cultures. CONCLUSION: The potential for a systemically applied drug to accumulate in tissue and negatively affect surrounding tissues and microstructures must be emphasized.en_US
dc.identifier.doi10.5137/1019-5149.JTN.24336-18.0
dc.identifier.endpage477en_US
dc.identifier.issn1019-5149
dc.identifier.issue4en_US
dc.identifier.pmid31124572en_US
dc.identifier.scopus2-s2.0-85068917407en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage470en_US
dc.identifier.trdizinid355915en_US
dc.identifier.urihttps://dx.doi.org/10.5137/1019-5149.JTN.24336-18.0
dc.identifier.urihttps://hdl.handle.net/20.500.12415/8588
dc.identifier.volume29en_US
dc.identifier.wosWOS:000473763100003en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherTURKISH NEUROSURGICAL SOCen_US
dc.relation.ispartofTURKISH NEUROSURGERYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzKY03715
dc.subjectCytotoxicityen_US
dc.subjectDabigatranen_US
dc.subjectDeep vein thrombosisen_US
dc.subjectIntervertebral disc cellsen_US
dc.subjectPrimary cell culturesen_US
dc.titleAre Intervertebral Disc Tissue Cells Damaged When Attempting to Prevent Thrombus Formation Using Dabigatran, A New Oral Anticoagulant?en_US
dc.typeArticle
dspace.entity.typePublication

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