Culturing, Freezing, Processing, and Imaging of Entire Organoids and Spheroids While Still in a Hydrogel

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dc.authoridKayalar, Özgecan/0000-0001-9107-2381en_US
dc.authoridAktas, Ranan/0000-0002-4474-7371en_US
dc.contributor.authorTok, Olgu Enis
dc.contributor.authorDemirel, Gamze
dc.contributor.authorSaatci, Yusuf
dc.contributor.authorAkbulut, Zeynep
dc.contributor.authorKayalar, Özgecan
dc.contributor.authorAktas, Ranan Gülhan
dc.date.accessioned2024-07-12T21:37:41Z
dc.date.available2024-07-12T21:37:41Z
dc.date.issued2022en_US
dc.department[Belirlenecek]en_US
dc.description.abstractOrganoids and spheroids, three-dimensional growing structures in cell culture labs, are becoming increasingly recognized as superior models compared to two-dimensional culture models, since they mimic the human body better and have advantages over animal studies. However, these studies commonly face problems with reproducibility and consistency. During the long experimental processes -with transfers of organoids and spheroids between different cell culture vessels, pipetting, and centrifuging -these susceptible and fragile 3D growing structures are often damaged or lost. Ultimately, the results are significantly affected, since the 3D structures cannot maintain the same characteristics and quality. The methods described here minimize these stressful steps and ensure a safe and consistent environment for organoids and spheroids throughout the processing sequence while they are still in a hydrogel in a multipurpose device. The researchers can grow, freeze, thaw, process, stain, label, and then examine the structure of organoids or spheroids under various high-tech instruments, from confocal to electron microscopes, using a single multipurpose device. This technology improves the studies' reproducibility, reliability, and validity, while maintaining a stable and protective environment for the 3D growing structures during processing. In addition, eliminating stressful steps minimizes handling errors, reduces time taken, and decreases the risk of contamination.en_US
dc.identifier.doi10.3791/64563
dc.identifier.issn1940-087X
dc.identifier.issue190en_US
dc.identifier.pmid36622008en_US
dc.identifier.scopus2-s2.0-85145275357en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.3791/64563
dc.identifier.urihttps://hdl.handle.net/20.500.12415/6875
dc.identifier.wosWOS:000920277000008en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherJournal of Visualized Experimentsen_US
dc.relation.ispartofJove-Journal of Visualized Experimentsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzKY04217
dc.titleCulturing, Freezing, Processing, and Imaging of Entire Organoids and Spheroids While Still in a Hydrogelen_US
dc.typeArticle
dspace.entity.typePublication

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