Rat penil erektil dokusunda medikal ve cerrahi kastrasyonun neden olduğu apoptozun karşılaştırmalı değerlendirilmesi
Küçük Resim Yok
Tarih
2012
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Maltepe Üniversitesi
Erişim Hakkı
Attribution-NonCommercial-NoDerivs 3.0 United States
info:eu-repo/semantics/openAccess
info:eu-repo/semantics/openAccess
Özet
Amaç: Siproteron asetat (CPA) ve cerrahi orşiektominin rat penil erektil dokusundaki apoptotik etkisini Bax proteinine bağlı olarak değerlendirmek. Yöntem: Postpubertal Wistar Albino ratlar üç gruba ayrıldı. İlk gruba (A) bilateral orşiektomi (n=10), ikinci gruba (B) haftada bir 14 gün süreyle 15 mg intramusküler CPA uygulaması (n=10) yapıldı. Üçüncü grup (C; sham opere) kontrol grubu (n=10) olarak alındı. Tüm ratlar postoperatif 14. günde sakrifiye edilip penil kavernöz cisimleri blok olarak alındı, Bax proteinine karşı oluşturulmuş anti-mouse IgG içerikli miyarla immünohistokimyasal olarak boyandı ve kavernözal alanlar değişik büyütmelerle mikroskop altında incelendi. Gruplar arasındaki Bax ekspresyonundaki farklılıklar one way ANOVA ile değerlendirildi. Bulgular: Proksimal, mid ve distal penil kesitler arasında apoptosis bakımından fark olmadığı görüldü. Cerrahi kastrasyon grubunun penil apoptotik derecesi CPA grubununkinden yüksek bulundu (p<0.001). Her iki gruptaki apoptoz derecesi kontrol grubununkinden yüksek idi (p<0.001). Sonuç: Medikal androjen deprivasyonunun da penil erektil dokuda apoptoza yol açtığı ancak bu etkinin cerrahi kastrasyona göre düşük olduğu gözlenmiştir.
Aim: To evaluate the apoptotic effect of Ciproterone acetate(CPA) and surgical orchidectomy on rat penile erectile tissue on the basis of Bax protein. Methods: Postpubertal Wistar Albino rats were divided in three groups: First group (A) underwent bilateral orchidectomy (n=10), second group (B) received 15 mg intramuscular CPA (n=10) every two weeks for 14 days. Third group (C; sham operated) was taken as control (n=10). All rats were sacrified at 14th day, their cavernous bodies were taken en bloc, stained immunohistochemically with a reagent containing anti-mouse IgG against Bax protein and, cavernous sections were evaluated under microscope with different magnifications. Bax expression differences among groups were analysed with one way ANOVA. Results: No apoptotic difference among proximal, mid and distal penile sections was found. The apoptotic index in surgically castrated group was superior compared to CPA treated group (p<0.001). Apoptotic indices in both groups were superior compared to control group (p<0.001). Conclusion: Although medical androgen deprivation also causes apoptosis on penile erectile tissue, its effect appears to be inferior compared to surgical castration.
Aim: To evaluate the apoptotic effect of Ciproterone acetate(CPA) and surgical orchidectomy on rat penile erectile tissue on the basis of Bax protein. Methods: Postpubertal Wistar Albino rats were divided in three groups: First group (A) underwent bilateral orchidectomy (n=10), second group (B) received 15 mg intramuscular CPA (n=10) every two weeks for 14 days. Third group (C; sham operated) was taken as control (n=10). All rats were sacrified at 14th day, their cavernous bodies were taken en bloc, stained immunohistochemically with a reagent containing anti-mouse IgG against Bax protein and, cavernous sections were evaluated under microscope with different magnifications. Bax expression differences among groups were analysed with one way ANOVA. Results: No apoptotic difference among proximal, mid and distal penile sections was found. The apoptotic index in surgically castrated group was superior compared to CPA treated group (p<0.001). Apoptotic indices in both groups were superior compared to control group (p<0.001). Conclusion: Although medical androgen deprivation also causes apoptosis on penile erectile tissue, its effect appears to be inferior compared to surgical castration.
Açıklama
Anahtar Kelimeler
Apoptoz, penil, kavernöz, erektil doku, cerrahi kastrasyon, siproteron asetat, Apoptosis, penile, cavernous, erectile tissue, surgical castration, ciproterone acetate
Kaynak
Maltepe Tıp Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
4
Sayı
1
Künye
Eyyüpoğlu, S. E., Sinanoğlu, O., Ekici, S., Aydın, M. ve Çek, M. (2012). Rat penil erektil dokusunda medikal ve cerrahi kastrasyonun neden olduğu apoptozun karşılaştırmalı değerlendirilmesi / Comparative evaluation of medical versus surgical castration on rat penile erectile tissue apoptosis. Maltepe Tıp Dergisi. 4(1), s .5-9.
