Examining the involvement of Slx5 in the apoptotic response to chronic activation of the spindle assembly checkpoint

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dc.contributor.authorAtalay, Pinar Buket
dc.contributor.authorCavusoglu, Elif Ergin
dc.contributor.authorAsci, Oyku
dc.contributor.authorAygunes, Duygu
dc.date.accessioned2024-07-12T21:54:08Z
dc.date.available2024-07-12T21:54:08Z
dc.date.issued2019en_US
dc.departmentMaltepe Üniversitesien_US
dc.description.abstractMicrotubule-targeting agents represent one of the most successful groups of anticancer drugs used in cancer therapy today. These drugs induce a prolonged mitotic arrest through chronic spindle assembly checkpoint (SAC) activation. Apoptosis, an outcome of the prolonged mitotic arrest, is the main mechanism by which these anticancer drugs kill cancer cells. However, not much is known about the mechanism that directs chronic SAC activation to apoptosis among other possible outcomes. The aim of this study is to investigate whether Slx5, a sumo-targeted ubiquitin E3 ligase, is involved in directing chronic SAC activation to apoptosis. We show that chronic SAC activation triggered by a 10-h nocodazole incubation leads to a prolonged mitotic arrest in the slx5 Delta strain similar to wild type (WT). However, the proportion of cells displaying apoptotic features such as nuclear fragmentation, DNA fragmentation, and reactive oxygen species (ROS) production were increased more in the WT strain during the chronic SAC activation compared to slx5 Delta, indicating that SLx5 may be involved in the chronic SAC-activation-apoptosis relation. We also showed that the possible role of Slx5 in the chronic SAC activation-apoptosis association was not through ubiquitin dependent degradation of 3 apoptosis-related and sumoylated candidate proteins.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [115Z157]en_US
dc.description.sponsorshipWe would like to thank Dr. Daniel J. Burke (North Carolina State University, College of Sciences, Department of Biological Sciences) for generously sending us the wild type TAP-tagged S. cerevisiae strains. This study was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) with the project number: 115Z157.en_US
dc.identifier.doi10.3906/biy-1812-46
dc.identifier.endpage197en_US
dc.identifier.issn1300-0152
dc.identifier.issn1303-6092
dc.identifier.issue3en_US
dc.identifier.pmid31320817en_US
dc.identifier.scopus2-s2.0-85071143276en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage189en_US
dc.identifier.trdizinid336043en_US
dc.identifier.urihttps://dx.doi.org/10.3906/biy-1812-46
dc.identifier.urihttps://hdl.handle.net/20.500.12415/8566
dc.identifier.volume43en_US
dc.identifier.wosWOS:000471268100004en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherTUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEYen_US
dc.relation.ispartofTURKISH JOURNAL OF BIOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKY03692
dc.subjectSaccharomyces cerevisiaeen_US
dc.subjectspindle assembly checkpointen_US
dc.subjectcanceren_US
dc.subjectprolonged mitotic arresten_US
dc.subjectapoptosisen_US
dc.titleExamining the involvement of Slx5 in the apoptotic response to chronic activation of the spindle assembly checkpointen_US
dc.typeArticle
dspace.entity.typePublication

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