Assessment of the neuroprotective effects of the acetylcholinesterase inhibitor huperzine A in an experimental spinal cord trauma model

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dc.contributor.authorAntar, Veysel
dc.contributor.authorBaran, Oguz
dc.contributor.authorYuceli, Sahin
dc.contributor.authorErdogan, Hakan
dc.contributor.authorAltintas, Ozge
dc.contributor.authorBaran, Gozde E.
dc.contributor.authorTasdemiroglu, Erol
dc.date.accessioned2024-07-12T21:51:07Z
dc.date.available2024-07-12T21:51:07Z
dc.date.issued2018en_US
dc.departmentMaltepe Üniversitesien_US
dc.description.abstractBACKGROUND: Spinal cord injury is nowadays still a challenging disease, and a treatment option aimed at the primary site of injury does not currently exist. Therefore, the management of acute spinal cord injury has recently focused on the reasons behind the aggravation of the initial insult through secondary mechanisms, and the search for pharmacological treatment protocols is generally aimed at reducing and minimizing the neural injury and neurological sequela. The secondary spinal cord injury usually develops following a primary lesion induced by spinal cord contusion and the emergence of apoptotic cells has been found to play an important role in the development of secondary injury. We propose that huperzine A may induce a significant reduction in the number of apoptotic cells because it possesses the ability to protect cells against glutamate, ischemia and staurosporine-induced cytotocity and apoptosis. METHODS: Huperzine A was administered intraperitoneally to male Wistar Albino rats (220-340 g of body weight) after moderate static clip compression (70 g for 60 s) of the spinal cord at T7 level. Neurological functions were assessed using the Basso-Beattle-Breshanan (BBB) motor rating scale until 3th and 7th days before perfusion, following which the spinal cord was harvested for histopathological examinations and apoptotic cell counts. RESULTS: Histopathological evaluations of the spinal cord of the control, trauma and huperzine A treated groups were evaluated. Control group showed normal neuronal and vascular structures of the spinal cord. However, in both trauma groups 3rd- and 7th-day perfusion showed extensive cavitation and hemorrhage, areas of necrosis and edema in gray. matter, and degeneration in motor neurons along with patchy areas of necrotic and apoptotic cells. In the group treated with huperzine A, an increased number of normal cells was observed, along with a lower number of necrotic cells, with a significant reduction in the apoptotic cells (P<0.01). The administration of huperzine A improved post-trauma motor performance. Furthermore, BBB scores of all groups showed that there was an improvement of locomotor abilities in the treatment group as compared with the control. CONCLUSIONS: When compared with controls, huperzine A treatment demonstrates a significant reduction in the number of apoptotic cells. In addition, the group treated with huperzine A showed significant and appreciable neurological improvement in rats.en_US
dc.identifier.doi10.23736/S0390-5616.16.03428-7
dc.identifier.endpage139en_US
dc.identifier.issn0390-5616
dc.identifier.issn1827-1855
dc.identifier.issue2en_US
dc.identifier.pmid26472141en_US
dc.identifier.scopus2-s2.0-85045923127en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage128en_US
dc.identifier.urihttps://dx.doi.org/10.23736/S0390-5616.16.03428-7
dc.identifier.urihttps://hdl.handle.net/20.500.12415/8241
dc.identifier.volume62en_US
dc.identifier.wosWOS:000445305500004en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherEDIZIONI MINERVA MEDICAen_US
dc.relation.ispartofJOURNAL OF NEUROSURGICAL SCIENCESen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzKY01993
dc.subjectSpinal cord injuriesen_US
dc.subjectNeuroprotectionen_US
dc.subjectAcetylcholinesteraseen_US
dc.subjectCholinesterase inhibitorsen_US
dc.subjectHuperzine Aen_US
dc.titleAssessment of the neuroprotective effects of the acetylcholinesterase inhibitor huperzine A in an experimental spinal cord trauma modelen_US
dc.typeArticle
dspace.entity.typePublication

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