Etanercept protects myocutaneous flaps from ischaemia reperfusion injury: An experimental study in a rat tram flap model

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dc.authorid0000-0001-8054-7740en_US
dc.authorid0000-0002-2591-9174en_US
dc.authorid0000-0002-9325-3757en_US
dc.contributor.authorErsoy, Burak
dc.contributor.authorCevik, Ozge
dc.contributor.authorCilingir, Ozlem Tugce
dc.date.accessioned2024-07-12T21:51:19Z
dc.date.available2024-07-12T21:51:19Z
dc.date.issued2016en_US
dc.departmentMaltepe Üniversitesien_US
dc.description.abstractBackground Being an inevitable component of free tissue transfer, ischemia-reperfusion injury tends to contribute to flap failure. TNF- is an important proinflammatory cytokine and a prominent mediator of the ischemia-reperfusion injury. Etanercept, a soluble TNF- binding protein, has shown anti-inflammatory and anti-apoptotic effects in animal models of renal and myocardial ischemia-reperfusion injury. We have designed an experimental study to investigate the effect of etanercept on myocutaneous ischemia-reperfusion injury on transverse rectus abdominis myocutaneous flap model in rats.Methods Twenty-four male Sprague-Dawley rats were divided into 3 groups: In group 1 (sham), the TRAM flap was raised and sutured back without further intervention. In group 2 (control), the flap was raised and the ischemia-reperfusion protocol was followed. In group 3, etanercept (10 mg/kg, i.v.) was administered 10 minutes before reperfusion. At the end of the reperfusion period, biochemical and histolopathological evaluations were performed on serum and tissue samples.Results In the etanercept group the IMA and 8-OHdG levels (p=0.005 and p=0.004, respectively) were found significantly lower, and the GSH and SOD levels (p=0.01 and p<0.001, respectively) significantly higher in comparison to the control group. The histopathological analysis has revealed a lower degree of hyalinization, degenerated muscle fibers and nuclear change in the etanercept group compared to the control group.Conclusion The results of our experimental study indicate that etanercept offers protection against ischemia-reperfusion injury in skeletal muscle tissue, enhancing the TRAM flap viability. The ability of etanercept to induce ischemic tolerance suggests that it may be applicable in free-flap surgery.en_US
dc.identifier.doi10.3109/2000656X.2016.1151437
dc.identifier.endpage215en_US
dc.identifier.issn2000-656X
dc.identifier.issn2000-6764
dc.identifier.issue4en_US
dc.identifier.pmid26950289en_US
dc.identifier.scopus2-s2.0-84961216627en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage208en_US
dc.identifier.urihttps://dx.doi.org/10.3109/2000656X.2016.1151437
dc.identifier.urihttps://hdl.handle.net/20.500.12415/8257
dc.identifier.volume50en_US
dc.identifier.wosWOS:000381362100004en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherTAYLOR & FRANCIS LTDen_US
dc.relation.ispartofJOURNAL OF PLASTIC SURGERY AND HAND SURGERYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzKY02019
dc.subjectEtanercepten_US
dc.subjectischemia-reperfusion injuryen_US
dc.subjectmyocutaneous flapen_US
dc.subjectoxidative stressen_US
dc.subjecttumor necrosis factor-alphaen_US
dc.titleEtanercept protects myocutaneous flaps from ischaemia reperfusion injury: An experimental study in a rat tram flap modelen_US
dc.typeArticle
dspace.entity.typePublication

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