Electron microscopic ımmunolocalization of basic fibroblast growth factor-like molecules in capillary endothelial cells.

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dc.authorid0000-0002-4474-7371en_US
dc.contributor.authorKayton, Robert J.
dc.date.accessioned2024-07-12T21:10:12Z
dc.date.available2024-07-12T21:10:12Z
dc.date.issued1998en_US
dc.departmentFakülteler, Tıp Fakültesien_US
dc.description.abstractBasic fibroblast growth factor (bFGF) is a potent angiogenic polypeptide. It promotes angiogenesis in vivo and in vitro by stimulating migration, proliferation and proteolytic activity of endothelial cells. Whereas several effects of exogenous bFGF on endothelial cells have been described, it has remained unclear how endogenous bFGF produced by vascular endothelial cells regulate angiogenesis. To further investigate functional implications of the distribution of bFGF, we undertook the present study. Our aims were (i) to identify the specific location of bFGF in endothelial cells using electron microscopy immunogold labeling technique (ii) to determine the distribution of bFGF in capillaries of different types of tissues. Tissue samples from sciatic nerve, hippocampus, adrenal gland and kidney of normal adult rats were fixed in 4% paraformaldehyde/1 to 5% glutaraldehyde and embedded in Spurr's resin. Ultrathin sections were labeled with either polyclonal (F3393-Sigma) or monoclonal antibodies (F6162-Sigma, C3316-ZymoGenetics) specific for bFGF using a two-step immunogold labeling method.en_US
dc.identifier.citationAktas, R. G. and Kayton, R. J. (1998). Electron Microscopic Immunolocalization of Basic Fibroblast Growth Factor-Like Molecules in Capillary Endothelial Cells. Microscopy and Microanalysis, Cambridge University Press. 4(S2), s. 1100–1101.en_US
dc.identifier.endpage1101en_US
dc.identifier.issn1435-8115
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1100en_US
dc.identifier.urihttps://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/electron-microscopic-immunolocalization-of-basic-fibroblast-growth-factorlike-molecules-in-capillary-endothelial-cells/FA943121E5F437EA067881F263A65B74
dc.identifier.urihttps://hdl.handle.net/20.500.12415/4209
dc.identifier.volume4en_US
dc.institutionauthorAktas, Ranan Gulhan
dc.language.isoenen_US
dc.publisherCambridge University Pressen_US
dc.relation.ispartofMicroscopy and Microanalysisen_US
dc.relation.isversionof10.1017/S1431927600025629en_US
dc.relation.publicationcategoryUluslararası Hakemli Dergide Makale - Kurum Öğretim Elemanıen_US
dc.rightsCC0 1.0 Universal*
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/*
dc.snmzKY02934
dc.titleElectron microscopic ımmunolocalization of basic fibroblast growth factor-like molecules in capillary endothelial cells.en_US
dc.typeArticle
dspace.entity.typePublication

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