Identification of microRNAs differentially expressed in prostatic secretions of patients with prostate cancer

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dc.contributor.authorGuzel, Esra
dc.contributor.authorKaratas, Omer F.
dc.contributor.authorSemercioz, Atilla
dc.contributor.authorEkici, Sinan
dc.contributor.authorAykan, Serdar
dc.contributor.authorYentur, Serhat
dc.contributor.authorCreighton, Chad J.
dc.contributor.authorIttmann, Michael
dc.contributor.authorOzen, Mustafa
dc.date.accessioned2024-07-12T21:49:46Z
dc.date.available2024-07-12T21:49:46Z
dc.date.issued2015en_US
dc.departmentMaltepe Üniversitesien_US
dc.description.abstractProstate cancer (PCa) is one of the leading causes of cancer deaths in men. Since there are limited treatment options available for the advanced tumors, there is an urgent need for novel diagnostic tools for PCa. Prostate secretion samples (PSS) from 23 PCa and 25 benign prostate hyperplasia (BPH) patients were obtained from Urology Department of Bagcilar Educational and Research Hospital (Istanbul). MicroRNA (miRNA) profiling of eight PSS (four from BPH, four from PCa patients) was performed using microarray. Four of significantly deregulated miRNAs were further confirmed using quantitative reverse-transcription PCR (qRT-PCR). Statistical analysis was performed using Student's t-test. ROC curves were plotted with SPSS-15.0. In this study, we aimed to identify a miRNA expression signature that could be used to distinguish PCa from BPH. MiRNA profiling of four PCa and four BPH patients with microarray revealed that miR-361-3p, miR-133b and miR-221 were significantly downregulated and miR-203 was upregulated in PSS of PCa patients. Further qRT-PCR analysis confirmed the altered expressions of these four miRNAs in PSS of 23 PCa and 25 BPH patients. Four miRNAs, together and individually have much power (AUC; 0.950) than PSA has (AUC; 0.463) to discriminate PCa from BPH patients. We have shown for the first time in the literature the presence of miRNAs in the PSS. We suggest PSS as a powerful non-invasive source for evaluation of prognosis in PCa, since prostate massages can be easily applied during routine examination. Our results showed that certain differentially expressed miRNAs in PSS could be used as diagnostics markers. What's new? The association of microRNAs (miRNAs) with cancer initiation, progression, and metastasis has fueled increasing interest in their potential as diagnostic and therapeutic markers. The current study aimed to identify an miRNA expression signature that could be used to distinguish prostate cancer from benign prostatic hyperplasia by using prostate secretions obtained from patients. MiRNAs were found to be present in the prostate secretion samples, with altered expression detected for four miRNAs in particular. The findings suggest that certain miRNAs may be powerful tools for aiding prostate cancer diagnosis.en_US
dc.description.sponsorshipScientific Research Projects of Istanbul University [21092]; The Scientific and Technological Research Council of Turkey (TUBITAK) [108S051]en_US
dc.description.sponsorshipGrant sponsor: Scientific Research Projects of Istanbul University; Grant number: 21092; Grant sponsor: The Scientific and Technological Research Council of Turkey (TUBITAK); Grant number: 108S051en_US
dc.identifier.doi10.1002/ijc.29054
dc.identifier.endpage879en_US
dc.identifier.issn0020-7136
dc.identifier.issn1097-0215
dc.identifier.issue4en_US
dc.identifier.pmid24976077en_US
dc.identifier.scopus2-s2.0-84918827158en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage875en_US
dc.identifier.urihttps://dx.doi.org/10.1002/ijc.29054
dc.identifier.urihttps://hdl.handle.net/20.500.12415/8077
dc.identifier.volume136en_US
dc.identifier.wosWOS:000346089900034en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherWILEY-BLACKWELLen_US
dc.relation.ispartofINTERNATIONAL JOURNAL OF CANCERen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKY01273
dc.subjectbenign prostatic hyperplasiaen_US
dc.subjectdiagnostic biomarkersen_US
dc.subjectmicroRNAen_US
dc.subjectprostate canceren_US
dc.titleIdentification of microRNAs differentially expressed in prostatic secretions of patients with prostate canceren_US
dc.typeArticle
dspace.entity.typePublication

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