PIWI interacting rna-651 inhibition transforms the genetic features of mcf-7 breast cancer cells

Basit Öğe Kaydı
dc.authorid0000-0003-3771-3277en_US
dc.contributor.authorÖner, Çağrı
dc.contributor.authorÇolak, Ertuğrul
dc.date.accessioned2024-07-12T21:10:32Z
dc.date.available2024-07-12T21:10:32Z
dc.date.issued2021en_US
dc.departmentFakülteler, Tıp Fakültesien_US
dc.description.abstractpiRNAs are novel members of small non-coding RNAs and have an impact on genetic and epigenetic mechanisms of cells. It was aimed to investigate the role of piR-651 on MCF-7 benign breast cancer cells by focusing on molecular characteristics. Anti-piR-651 was transfected and effects of piR-651 on proliferation, adhesion, and motility of MCF-7 cells were detected after the 24th, 48th, and 72nd hour. Gene expressions of piR-651, Ki-67, MMP-2, ER?, HIF-1?, and hTERT were determined by using RT-PCR. piR-651 inhibition caused the decrease of proliferation, adhesion (p < 0.001), and motility of MCF-7 cells. The efficiency of anti-piR-651 transfection supported the determination of the decrease of piR-651 expression after transfection after the 48th hour (p < 0.001). AntipiR-651 transfection caused the downregulation of Ki-67, MMP-2, ER?, HIF-1?, and hTERT gene expressions after the 48th hour (p < 0.001). In MCF-7 cells, piR-651 inhibition can change both cellular characteristics and gene expressions which were related to these characteristics. piR-651 inhibition causes the decrease of both proliferation and adhesion of cells, which are especially important cellular marks of MCF-7 cells. These results have shed light on whether we can mitigate the effects of cancer cells via piRNA inhibition. The absence of piRNA expression caused the change of fate of benign breast cancer cells by decreasing malign features of MCF-7 cells.en_US
dc.identifier.citationÖner, Ç. ve Çolak, E. (2021). PIWI interacting rna-651 inhibition transforms the genetic features of mcf-7 breast cancer cells. Oncologie, Tech Science Press. 23(3), s. 393-407.en_US
dc.identifier.doi10.32604/Oncologie.2021.016958
dc.identifier.endpage407en_US
dc.identifier.issn1765-2839
dc.identifier.issue3en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage393en_US
dc.identifier.urihttps://www.techscience.com/oncologie/v23n3/44594
dc.identifier.urihttps://doi.prg/10.32604/Oncologie.2021.016958
dc.identifier.urihttps://hdl.handle.net/20.500.12415/4260
dc.identifier.volume23en_US
dc.identifier.wosWOS:000701857100007en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Science
dc.institutionauthorÖner, Çağrı
dc.language.isoenen_US
dc.publisherTech Science Pressen_US
dc.relation.ispartofOncologieen_US
dc.relation.publicationcategoryUluslararası Hakemli Dergide Makale - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKY03160
dc.subjectAdhesionen_US
dc.subjectbreast canceren_US
dc.subjectmotilityen_US
dc.subjectPIWI interacting RNAsen_US
dc.subjectpiR-651en_US
dc.subjectproliferationen_US
dc.titlePIWI interacting rna-651 inhibition transforms the genetic features of mcf-7 breast cancer cellsen_US
dc.typeArticle
dspace.entity.typePublication

Dosyalar

Koleksiyon

Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu