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Yayın Assessment of the impact of Curcumin on cell cultures derived from the primary intervertebral disc tissue in humans / İnsanlarda primer intervertebral disk dokusundan elde edilen hücre kültürleri üzerinde Curcumin'in etkisinin değerlendirilmesi(T.C.Maltepe Üniversitesi, 2023) Albayrak, Mehmet; Yilmaz, İbrahim; Yüzbasi, Muharrem Furkan; Akalan, Hande; Yasar Şirin, Duygu; Karaarslan, Numan; Özbek, Hanefi; Ateş, ÖzkanAim: Degenerative disc disease in the lumbar spine is widely observed. Degenerative disc diseases are among the causes of low back pain in older age. Modern drug discovery studies have aimed to identify potential molecules that target multiple pathways with a safer profile against degeneration. This study aimed to evaluate the effects of curcumin, a natural phenolic compound, on primary cell cultures prepared using intervertebral disc (IVD) tissues resected during the surgeries of patients with lumbar disc herniation. Material and Methods: Primary cell cultures were prepared using human IVD tissues of eight patients. Untreated groups served as the control and curcumin-treated groups as the study sample. Invitro cytotoxicity analyses were performed in all groups. Acridine orange (AO)/propidium iodide (PI) and Janus Green B staining were performed to evaluate cell surface morphologies. One-way analysis of variance and Tukey HSD, a multiple comparison test, were used to assess the obtained data. Results: Proliferation slightly increased as of 24 h in the curcumintreated samples, but decreased in the 48 and 72 hour curcumintreated samples compared to the control samples. The obtained results were statistically significant (p<0.05). Additionally no cytotoxicity was observed according to morphological evaluations. Conclusion: This research is an in-vitro experimental study. However, this natural and non-toxic pleiotropic agent can be targeted to cell-damaged sites through appropriate drug delivery systems. It may thus be a safe treatment option for the regeneration of degenerated lumbar IVD cells in the future.Yayın Evaluation of the effect of apixaban on the primary intact intervertebral disc cell cultures(2019) Şimşek, Abdullah Talha; Özbek, Hanefi; Şirin, Duygu Yaşar; Yılmaz, İbrahim; Ateş, Özkan; Akgün, Feride Sinem; Kaplan, NecatiAim: Apixaban is a frequently preferred pharmacological agent in clinics to prevent deep vein thrombosis and pulmonary embolism.Such new oral anticoagulants may cause hemorrhage’s in tissues and/or organs or may cause gastrointestinal symptoms withoutbleeding. It is also reported in the literature that it may lead to mental disorders, unwanted disorders in the urinary tract and skeletalmuscle system. However, when the literature is examined, there are no studies, which are of high-evidential value, evaluating theefficacy of apixaban on healthy, intact intervertebral disc tissue, and matrix-like structures. In this pharmaco-molecular study, itwas aimed to investigate the effects of a new oral anticoagulant agent containing the active ingredient apixaban on the intactintervertebral disc tissue cells, extracellular matrix (ECM) structure and to evaluate its positive and / or negative effects on geneexpressions of cartilage oligo matrix protein (COMP), chondroadherin (CHAD), and Matrix Metalloproteinase (MMP)s.Material and Methods: The primary cell cultures were prepared from the intact tissues of the patients with the traumatic intervertebraldisc herniation. Apixaban was administered to the cultures and molecular analyses were performed for 21 days. The data obtainedfrom the apixaban-administered and non-apixaban-administered samples were evaluated statistically and the significance valuewas accepted as P <0.05.Results: The changes were observed in the cell proliferation and the expressions of the mentioned genes in the apixabanadministered group. The suppression of COMP value and the increase in MMP-13 value may be indicative of the development ofmatrix degeneration in the apixaban-administered group, compared to the non-drug-administered control group.Conclusion: The selectivity is one of the most important features of the drugs. However, it should not be forgotten that no drug willonly produce the desired effect.Yayın Evaluation of treatment of patients with SARS-CoV-2 in line with literature / SARS-CoV-2 tanısı ile takip edilen olguların tedavisinin literatür eşliğinde sistematik olarak değerlendirilmesi(Maltepe Üniversitesi, 2020) Karaarslan, Numan; Doğan, Mustafa; Yılmaz, İbrahim; Bilir, Bülent; Hacıoğlu, Fatma; Onar, Lütfi; Özbek, HanefiAims: The present article aimed to evaluate the drug therapy applied to patients who were followed up for SARS-CoV-2, and who were tested positive and negative 2019-nCov. Material and Methods: A comprehensive and systematic literature search of numerous electronic databases regarding patients SARS-CoV-2 was performed. Results: Patients with 2019-nCoV can be treated with ceftriaxone, moxifloxacin, oseltamivir, hydroxychloroquine, and patients with poor prognosis can also be given lopinavir/ritonavir. Conclusions: Not only rRT-qPCR test results and CT findings but also clinical evidence should be considered for the diagnosis of SARS-CoV-2.Yayın Investigation of the effect of dipyrone on cells isolated from intervertebral disc tissue(Spandidos Publ Ltd, 2019) Akgün, Feride Sinem; Sirin, Duygu Yasar; Yılmaz, Ibrahim; Karaarslan, Numan; Özbek, Hanefi; Simsek, Abdullah Talha; Kaya, Yasin EmreThe present study aimed to evaluate the effects of dipyrone, an indispensable analgesic, anti-pyretic and anti-spasmodic used in emergency departments, on nucleus pulposus and annulus fibrosus cells in vitro. After surgical biopsy, primary cell cultures were prepared from intact intervertebral disc tissues. Dipyrone was administered to the cultures in the experimental groups except for the control group. The data obtained were statistically evaluated. The proliferation was identified to be suppressed via MTT analysis. The gene expression profile of the intervertebral disc cells in the dipyrone-treated groups was significantly changed. The expression of chondroadherin, cartilage oligo matrix protein, interleukin-1 beta and metalloproteinase (MMP)-19 genes were decreased, but MMP-13 and MMP-7 genes expressions were increased, as determined via reverse transcription-quantitative PCR. AO/PI staining revealed that no apoptotic or other type of cell death was detectable after administration of dipyrone does not mean that the drug is innocuous. The occurrence of cellular senescence and/or the halt of cell proliferation may also be important mechanisms underlying the adverse inhibitory effects of dipyrone. Therefore, prior to administering dipyrone in clinical practice, all possible adverse effects of this drug should be considered.Yayın Systematic Evaluation of Desmopressin Administered to Patients with Aneurysmal Subarachnoid Hemorrhage in the Light of the Literature(Turkish Neurosurgical Soc, 2020) Karaarslan, Numan; Yılmaz, Ibrahim; Akgün, Feride Sinem; Caliskan, Tezcan; Özbek, Hanefi; Ates, ÖzkanAIM: To discuss the management of patients with the syndrome of inappropriate antidiuretic hormone secretion (SIADH) developing after subarachnoid hemorrhage, in a comparative manner in the light of the literature. MATERIAL and METHODS: Without country or language restrictions, articles with high evidential value found in electronic databases were compared to our patients' data. RESULTS: After the literature review, three articles were included for systematic evaluation. Desmopressin was administered to the patients for the treatment of hyponatremia, volume contraction, and negative sodium balance caused by SIADH. However, it was not used for preventing re-bleeding. CONCLUSION: To prevent the development of this complication (SIADH), the use of desmopressin, an analogue of vasopressin, is important in routine clinical practice.
