Effects of some drugs on enzyme activity of catalase from bovine liver

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dc.authorid0000-0003-0552-085Xen_US
dc.authorid0000-0003-2099-7277en_US
dc.authorid0000-0003-2101-1334en_US
dc.contributor.authorAlkan, Salih
dc.contributor.authorSavran, Ali
dc.contributor.authorDemir, Halit
dc.contributor.authorCeylan, Hasan
dc.contributor.authorCeylan, Hasan
dc.date.accessioned2024-07-12T20:35:58Z
dc.date.available2024-07-12T20:35:58Z
dc.date.issued2006en_US
dc.departmentFakülteler, Eğitim Fakültesi, İlköğretim Matematik Öğretmenliği Bölümüen_US
dc.description.abstractThe effects of gentamicin sulphate, acetyl salicylic acid, ampicillin sodium, paracetamol, potassium penicillin and augmentin were investigated on the in-vitro enzyme activity of catalase. Catalase (CAT:EC1.11.1.6) was purified from bovine liver by a simple and rapid method. The purification process was done by 2´,5´-ADP sepharose 4B affinity chromatography. Although the purified enzyme showed a tetrameric band on sodium dodecyl sulphate polyacryilamide gel electrophrosis but bovine liver showed a one band. The enzyme activity was measured spectrophotometrically at 240 nm, according to the method of Aebi. From these six drugs, paracetamol, potassium penicillin and augmentin inhibited the activity of the purified enzyme; gentamicin sulphate, acetyl salicylic acid and ampicillin sodium showed little effect on the enzyme activity. The I50 values for these three drugs were as 4.6, 0.35 and 0.49 mM, respectively. The Ki constants were 20, 25 and 25 mM, respectively and they were competitive inhibitors.en_US
dc.identifier.citationAlkan, S., Savran, A., Demir, H. ve Ceylan, H. (2006). Effects of some drugs on enzyme activity of catalase from bovine liver. Asian Journal of Chemistry. 18(1), s. 601-607.en_US
dc.identifier.endpage607en_US
dc.identifier.issn0975-427X
dc.identifier.issue1en_US
dc.identifier.startpage601en_US
dc.identifier.urihttp://www.asianjournalofchemistry.co.in/User/SearchArticle.aspx?Volume=18&Issue=1&Article=&Criteria=
dc.identifier.urihttps://hdl.handle.net/20.500.12415/995
dc.identifier.volume18en_US
dc.language.isoenen_US
dc.publisherAsian Publication Corporationen_US
dc.relation.ispartofAsian Journal of Chemistryen_US
dc.relation.publicationcategoryUluslararası Hakemli Dergide Makale - Kurum Öğretim Elemanıen_US
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
dc.snmzKY00358
dc.subjectCatalaseen_US
dc.subjectBovine liveren_US
dc.subjectGentamicin sulphateen_US
dc.subjectAcetyl salicylic aciden_US
dc.subjectAmpicillin sodiumen_US
dc.subjectParacetamolen_US
dc.subjectPotassium penicillinen_US
dc.subjectAugmentinen_US
dc.titleEffects of some drugs on enzyme activity of catalase from bovine liveren_US
dc.typeArticle
dspace.entity.typePublication

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