Infusion or Decoction Extracts ofHelianthus annuusLeaves: Potential Inhibitors for QS system and Biofilm Formation in Pseudomonas aeruginosa

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dc.contributor.authorYıldız, Ayla
dc.contributor.authorSesal, Cenk
dc.contributor.authorGökalsın, Barış
dc.contributor.authorBalkan, Arhun Ali
dc.contributor.authorBerber, Didem
dc.date.accessioned2024-07-12T22:04:12Z
dc.date.available2024-07-12T22:04:12Z
dc.date.issued2020en_US
dc.description.abstractPseudomonas aeruginosais one of the drug-resistant opportunistic pathogenswith the ability to form biofilm and to produce a number of virulence factors via Quorum Sensing (QS) regulation. Most researchers have focused on QS inhibition to overcome the drug resistance problem.QS inhibitormolecules are investigated from natural resources.In the present study, anti-QS activities of ethyl acetate extracts of decoction and infusion samples from Helianthus annuusleaveswere tested on biosensor strains of P.aeruginosa(lasB-gfp, rhlA-gfp andpqsA-gfp), as well as anti-biofilm activities on PAO1wild type. H.annuusleaf sampleswere firstly infused or decocted and then extracted with ethyl acetate. Theefficacies ofinfusion or decoction extracts were examinedat the concentrationsof 240, 120,and60?g/mlin 96-well microplatesand evaluated in Citation 3 multimode microplate reader (Biotek). The inhibition rates of decoction extracts were recorded as 70.61% for las, 44.09% for rhland 83.77% for pqssystem at 240 ?g/ml. The biofilm inhibition percentages of the extracts were determined to be 50.82% (±1.36). Moreover, inhibition rates for infusion extracts were detected as 62.08% for las, 45.15% for rhland 77.79% for pqs, and 53.88% (±3.94) for biofilm formation. In conclusion, the potential efficacies of the extracts of decocted or infused H.annuusleaves were demonstratedon QS system and biofilmformation of P.aeruginosa. However, there is a need for more detailed investigations and determination of the active substances that have QSI and anti-biofilm effect.en_US
dc.identifier.doi10.7240/jeps.741137
dc.identifier.endpage506en_US
dc.identifier.issn2636-8277
dc.identifier.issue4en_US
dc.identifier.startpage499en_US
dc.identifier.trdizinid413178en_US
dc.identifier.urihttps://doi.org/10.7240/jeps.741137
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/413178
dc.identifier.urihttps://hdl.handle.net/20.500.12415/9579
dc.identifier.volume32en_US
dc.indekslendigikaynakTR-Dizin
dc.language.isoenen_US
dc.relation.ispartofInternational journal of advances in engineering and pure sciences (Online)en_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKY04874
dc.titleInfusion or Decoction Extracts ofHelianthus annuusLeaves: Potential Inhibitors for QS system and Biofilm Formation in Pseudomonas aeruginosaen_US
dc.typeArticle
dspace.entity.typePublication

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