The effect of glutathione preconditioning on heme oxygenase-1 system established by whey protein feeding on hepatocellular injury in a rat hepatic normothermic i/r injury model [Siçanlarda whey proteini ile oluşturulan glutatyon önkoşullandirmasinin karaci?er sicak iskemi/reperfüzyon hasarinda hem oksijenaz-1 sistemi üzerine etkisi]

Basit Öğe Kaydı
dc.contributor.authorManukyan, Manuk Norayık
dc.contributor.authorYavuz Y.
dc.contributor.authorErbarut I.
dc.contributor.authorÖgünç A.V.
dc.contributor.authorAtaizi-Çelikel Ç.
dc.contributor.authorSüha Yalçin A.
dc.contributor.authorGüllüoglu B.M.
dc.date.accessioned2024-07-12T21:56:44Z
dc.date.available2024-07-12T21:56:44Z
dc.date.issued2008en_US
dc.departmentMaltepe Üniversitesien_US
dc.description.abstractPurpose: Oxygen radicals which are produced during reperfusion phase play a central role for hepatocellular damage during ischemia/reperfusion (l/R) injury. Antioxidant strategies appear a promising approach to prevent I/R injury. In the present study, the effect of glutathione (GSH) preconditioning established by whey protein feeding on hepatocellular injury markers was assessed in a rat hepatic normothermic l/R injury model. The interaction between intracellular GSH content and heme oxygenase-1 [HO-1] enzyme expression was also determined in order to understand the level of upgrading of HO-1 system in a high GSH content environment after preconditioning. Material and Methods: Livers of female Sprague-Dawley rats were subjected to 45/45 minutes of normothermlc l/R injury. A group of rats were fed by standard chow for a three week duration before I/R procedure (CONTROL l/R: n«16), whereas another group was fed by "whey" protein for preconditioning (WHPR l/R; n=16) during the same period. Two SHAM groups were constituted accordingly but rats were not subjected to l/R injury (STD SHAM and WHEY SHAM; n=6 in each). Following 45 minutes of reperfusion, serum transaminase levels as well as GSH, malondialdehyde (MDA) levels, apoptotic index (Al) and HO-1 gene expression In liver tissue were determined. Half of the animals In both injury groups were surveilled for a week without sampling. Results: Intracellular GSH levels in WHEY SHAM group were significantly higher than those of STD SHAM group which indicated a successful preconditioning. Preconditioning by "whey" protein feeding prior to l/R injury (WHPR l/R) significantly ameliorated the increased levels of serum transaminase as well as liver MDA levels and Ai in CONTROL l/R group. Survival of the rats In both injury groups were not different. "Whey" preconditioning also significantly increased GSH levels, whereas HO-1 expression was lower when compared to rats subjected to l/R Injury without preconditioning. Conclusion: Feeding with "whey" protein successfully resulted high GSH content in rat liver. Whey preconditioning ameliorated hepatic l/R Injury. HO-1 enzyme seems to have a less important role in antioxidant defense system when GSH production was induced prior to l/R injury.en_US
dc.identifier.endpage144en_US
dc.identifier.issn1300-0705
dc.identifier.issue3en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage137en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12415/8599
dc.identifier.volume24en_US
dc.language.isotren_US
dc.relation.ispartofTurkish Journal of Surgeryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzKY03747
dc.subjectGlutathioneen_US
dc.subjectHeme oxygenase-1en_US
dc.subjectIschemia/reperfusionen_US
dc.subjectLiveren_US
dc.subjectWheyen_US
dc.titleThe effect of glutathione preconditioning on heme oxygenase-1 system established by whey protein feeding on hepatocellular injury in a rat hepatic normothermic i/r injury model [Siçanlarda whey proteini ile oluşturulan glutatyon önkoşullandirmasinin karaci?er sicak iskemi/reperfüzyon hasarinda hem oksijenaz-1 sistemi üzerine etkisi]en_US
dc.typeArticle
dspace.entity.typePublication

Dosyalar

Koleksiyon

Scopus İndeksli Yayınlar Koleksiyonu