Effects of different glucose concentrations on the leptin signaling pathway in MCF-7 and T47D breast cancer cells

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dc.contributor.authorDemirel, Pınar Buket
dc.contributor.authorCleary, Margot
dc.contributor.authorTuna, Bilge Güvenç
dc.contributor.authorÖzorhan, Ümit
dc.contributor.authorDoğan, Soner
dc.date.accessioned2024-07-12T22:04:07Z
dc.date.available2024-07-12T22:04:07Z
dc.date.issued2019en_US
dc.description.abstractAim: Leptin activates multiple intracellular signaling pathways, including JAK/STAT, by binding to its receptor. Leptin is also animportant regulator of glucose homeostasis. Although both glucose and leptin increase breast cancer cell proliferation in vitro,whether the enhancing effect of glucose on the proliferation of breast cancer cells is mediated by the leptin signaling pathway isnot known. The aim of this study was to investigate the effect of different glucose concentrations on the leptin signaling pathway inMCF-7 and T47D breast cancer cells.Material and Methods: MCF-7 and T47D cell proliferation in different glucose concentrations (2.5 mM, 5 mM, 25 mM, or 50 mM)were assayed using CCK-8 assay. Leptin, leptin receptors (ObR, ObRb) as well as STAT3 mRNA and protein levels in both cell lines indifferent glucose concentrations were examined by RT-PCR and western blot, respectively.Results: Incubation in 2.5 mM, 5 mM, 25 mM, or 50 mM glucose for 72h significantly increased the proliferation of both MCF-7 andT47D cells compared to 0 mM glucose incubated cells (P<0.001). mRNA levels of leptin, ObR, ObRb or STAT3 in 2.5 mM, 5 mM,25 mM, or 50 mM glucose incubated cells were not significantly different in both cell lines compared to 0 mM (p>0.05). However,ObR protein levels in MCF-7 cells incubated in 25 mM glucose was significantly lower compared to 0 mM glucose by western blot(p<0.05).Conclusion:Our data suggest that the enhancing effect of glucose on breast cancer cell proliferation is not mediated by the JAK/STAT pathway.en_US
dc.identifier.doi10.5455/annalsmedres.2019.09.542
dc.identifier.endpage2971en_US
dc.identifier.issn2636-7688
dc.identifier.issue12en_US
dc.identifier.startpage2966en_US
dc.identifier.trdizinid359221en_US
dc.identifier.urihttps://doi.org/10.5455/annalsmedres.2019.09.542
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/359221
dc.identifier.urihttps://hdl.handle.net/20.500.12415/9542
dc.identifier.volume26en_US
dc.indekslendigikaynakTR-Dizin
dc.language.isoenen_US
dc.relation.ispartofAnnals of Medical Researchen_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKY04824
dc.titleEffects of different glucose concentrations on the leptin signaling pathway in MCF-7 and T47D breast cancer cellsen_US
dc.typeArticle
dspace.entity.typePublication

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