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Yayın Discrimination of dwarf honey bee (Apis florea, Fabricius 1876) populations in Iran using elliptic Fourier wing cell shape analysis(TAYLOR & FRANCIS LTD, 2018) Koca, Ayca Ozkan; Moradi, Mohammed G.; Deliklitas, Ozlem; Ucan, Amine; Kandemir, IrfanWe investigated cell shape differentiation among Apis florea populations distributed in four states (Hormuzgan-Bushehr-Khuzestan-Ilam states) of Iran using elliptic Fourier analysis (EFA - outline-based geometric morphometric analysis) on all forewing cells. Outline-based analysis was performed on all closed cells on the forewings of 114 A. florea colonies from 13 locations in Iran. According to the statistical analysis of each cell shape, the most efficient cells to discriminate populations were 2nd cubital, 3rd submarginal and marginal cells. Discriminant Function Analysis of Fourier coefficients (FCs) for each cell resulted in two clusters: southern A. florea populations from lower latitude (Hormuzgan state); and the rest of the populations from higher latitude (Ilam, Khuzestan, Bushehr state) with different differential discrimination values (1st submarginal cell=75.4%, 2nd submarginal cell=86.0%, 1st medial cell and 2nd medial cell=86.8%, marginal cell=88.6%, 3rd submarginal cell=90.4%, 2nd cubital cell=92.1%). This study showed that EFA has been functional and effective for distinguishing A. florea populations based on the wing cell shapes. The outline-based geometric morphometric applied in our study has been as successful as the other morphometric approaches such as standard morphometric and landmark-based geometric morphometric in previous studies. Se investigo la diferenciacion de las celdillas alares en poblaciones de Apis florea, distribuidas en cuatro estados (Hormuzgan, Bushehr, Khuzestan e Ilam) de Iran usando el analisis eliptico de Fourier (EFA, analisis de morfometria geometrica basada en trazos) de todas las celdillas de las alas anteriores. El analisis basado en trazos fue llevado a cabo en todas las celdillas cerradas de las alas anteriores de 114 colonias de A. florea en 13 localizaciones distintas de Iran. Segun el analisis estadistico de la forma de cada celdilla, las celdillas mas adecuadas para discriminar las poblaciones son la segunda cubital, la tercera submarginal y la celdilla marginal. Los coeficientes del analisis discriminativo de funciones de Fourier (FCs) para cada celdilla formaron dos clusteres: las poblaciones de A. florea del sur, de latitudes bajas (estado de Hormuzgan); y el resto de poblaciones de latitudes altas (Ilam, Khuzestan y Bushehr) con un diferente valor de discriminacion diferencial (1 celdilla submarginal = 75.4%, 2 celdilla submarginal = 86.0%, 1 celdilla media y 2 celdilla media = 86.8%, celdilla marginal = 88.6%, 3 celdilla submarginal = 90.4%, 2 celdilla cubital= 92.1%). Este estudio muestra que el EFA es util y efectivo para distinguir las poblaciones de A. florea basandose en la forma de sus celdillas alares. El analisis de la morfometria geometrica basada en trazos aplicado en nuestro estudio ha sido tan exitoso como otros metodos morfometricos utilizados en estudios previos, como la morfometria estandar y la morfometria geometrica basada en puntos.Yayın Population structure and pattern of geographic differentiation of Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae) in Turkey(John Wiley & Sons Ltd, 2022) Özkan Koca, Ayca; Berkcan, Salih B.; Lacin Alas, Burcu; Kandemir, IrfanBACKGROUND The Colorado potato beetle (CPB) is the most harmful pest of potato in potato cultivation regions globally. Although it is an economically important agricultural pest, the population structure and colonization route of this species in Turkey are uncertain. We used microsatellite and mitochondrial DNA (mtDNA) markers to obtain information about the population source, structure and bio-invasion route of CPB populations in Turkey. RESULTS The common single mtDNA haplotype in European CPB populations was obtained in all Turkish CPB populations based on mtDNA data analysis. However, microsatellites revealed a low level of genetic variation in CPB populations. The results of microsatellite analysis [factorial correspondence analysis (FCA), Bayesian analysis of genetic population structure (BAPS), unweighted pair group method with arithmetic mean (UPGMA) dendrogram, F-statistics and Nei's distances] indicated three groups for invasive CPB: Thrace-Marmara and Aegean; Black Sea, Central Anatolia and Mediterranean; Northeastern Anatolia. Region-specific alleles have been identified in regions, where commercial potato cultivation and insecticide use are intensive. CONCLUSION The detection of a single fixed European haplotype in all Turkish populations has proved that CPB in Turkey originated from Europe as a result of a founder event occurred in European populations. Low genetic variation was due to the short time period since the spread of CPB from America to Europe. The highest number of private alleles were found in the top commercial potato cultivation region-Central Anatolia from where the CPB populations spread to other parts of Turkey.