Yazar "Dogan, Soner" seçeneğine göre listele

Listeleniyor 1 - 3 / 3
  • Küçük Resim Yok
    Yayın
    Effects of Chronic and Intermittent Calorie Restriction on Adropin Levels in Breast Cancer
    (ROUTLEDGE JOURNALS, TAYLOR & FRANCIS LTD, 2017) Tuna, Bilge G.; Atalay, Pinar B.; Altunbek, Mine; Kalkan, Batuhan M.; Dogan, Soner
    Adropin is a peptide hormone that has been implicated in insulin resistance and as a potential regulator of growth. The aim of this study is to determine the effect of calorie restriction on circulating levels of adropin in the MMTV-TGF breast cancer mouse model and investigate the effects of adropin peptide on the viability of MCF-7 and MDA-231 breast cancer cells in culture. Ten-week-old mice were assigned to either ad libitum-fed (AL), chronic calorie-restricted, or intermittent calorie-restricted groups. Concentrations of serum adropin were measured using an enzyme-linked immunosorbent assay. Results showed an inverse correlation between serum adropin levels and mouse age that was attenuated by calorie restriction. In the AL group the level of adropin was significantly lower at week 50 compared to levels at week 10. However, among the calorie-restricted groups, serum levels of adropin remained high at week 50. The cell-line-specific effects were observed after treatment of cancer cell lines with a series of adropin concentrations (5, 10, 25, 50ng/mL). Flow cytometry analysis showed that MCF-7 cells entered the early phase of apoptosis after treatment with 50ng/mL for 24h. Adropin may be involved in the protective effects that calorie restriction has on breast cancer risk.
  • Küçük Resim Yok
    Yayın
    Kromozom anomalilerinde iğ ipliği kontrol noktası: saccharamyces cerevisiae modelinde six5'in kemoterapötik hedef olarak değerlendirilmesi
    (2016) İsan, Hatice; Demirel, Pınar Buket; Dogan, Soner
    [Abstract Not Available]
  • Küçük Resim Yok
    Yayın
    Roles of adiponectin and leptin signaling-related microRNAs in the preventive effects of calorie restriction in mammary tumor development
    (Canadian Science Publishing, 2021) Dogan, Soner; Cicekdal, Münevver B.; Özorhan, Umit; Karabiyik, Goktug; Kazan, Busra T.; Ekici, Isin D.; Yılmaz, Bayram
    Calorie restriction (CR) is suggested to prevent the development of mammary tumors (MTs); however, the mechanism remains to be clarified. We aimed to determine the microRNA (miRNA) profile in mice applied to 2 different CR protocols; chronic (CCR) and intermittent (ICR) and follow the MT development. In addition, the roles of miRNAs involved in adiponectin and/or leptin signaling pathways were investigated. Mice were divided into 3 groups: ad-libitum (AL), CCR, or ICR, which comprised 3 weeks of AL feeding followed by I week of 60% CR in a cyclic manner. Blood and tissue collection were performed at weeks 10,17/18, 49/50 and 81/82. Long-term CCR provided better protection compared with ICR for MT development with a delay in the MT occurrence. Adiponectin expression in mammary fat pad were significantly higher in CCR group compared with AL. Using GeneChip Array, 250 of 3195 miRNAs were differentially expressed among the dietary groups. Thirteen of 250 miRNAs were related to adiponectin and/or leptin signaling genes. Results were verified by reverse transcription polymerase chain reaction. Specifically, miR-326-3p, miR-500-3p and miR-129-5p, which are related to adiponectin and/or leptin signaling, may play important roles in the preventive effects of CR in MT development and in ageing. Thus, these miRNAs might be putative biomarkers to target for diagnostic and treatment purposes. Novelty: Type of CR and micro RNA interaction is related to ageing. miR-326-3p, miR-500-3p and miR-129-5p expression levels were differentially expressed in MT development and in ageing. The genes associated with adiponectin and/or leptin signaling pathways are regulated by certain miRNAs in the protective effects of CR.